| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 03-Mar-2001 |  Franco Mostert | Lions | South Africa | 27-Nov-1990 | 10(97) |
| 23-Feb-2001 | Aaron Mauger | Crusaders | New Zealand | 29-Nov-1980 | 20(86) |
| 21-Apr-2001 | JP Nel | Bulls | South Africa | 09-Jan-1981 | 20(102) |
| 24-Feb-2001 | Jerry Collins | Hurricanes | New Zealand | 04-Nov-1980 | 20(112) |
| 14-Apr-2001 | Junior Pelesasa | Queensland Reds | Australia | 17-Nov-1980 | 20(148) |
| 03-Mar-2001 | Adrian Jacobs | Bulls | South Africa | 14-Aug-1980 | 20(201) |
| 02-Mar-2001 | Mils Muliaina | Blues | New Zealand | 31-Jul-1980 | 20(214) |
| 23-Feb-2001 | George Smith | Brumbies | Australia | 17-Jul-1980 | 20(221) |
| 24-Feb-2001 | David Lyons | New South Wales | Australia | 16-Jun-1980 | 20(253) |
| 27-Apr-2001 | Tui Talaia | New South Wales | Australia | 02-Jun-1980 | 20(329) |
| 24-Mar-2001 | Sam Harris | New South Wales | Australia | 30-Jan-1980 | 21(54) |
| 24-Feb-2001 | Steve Kefu | Queensland Reds | Australia | 06-Dec-1979 | 21(81) |
| 23-Feb-2001 | Orene Ai'I | Blues | New Zealand | 23-Nov-1979 | 21(93) |
| 10-Mar-2001 | Carl Hayman | Highlanders | New Zealand | 14-Nov-1979 | 21(117) |
| 24-Feb-2001 | Jonathan West | New South Wales | Australia | 23-Oct-1979 | 21(125) |
| 24-Feb-2001 | Rodney So'oialo | Hurricanes | New Zealand | 01-Oct-1979 | 21(147) |
| 24-Feb-2001 | Phil Waugh | New South Wales | Australia | 22-Sep-1979 | 21(156) |
| 03-Mar-2001 | Joshua Blackie | Highlanders | New Zealand | 03-Aug-1979 | 21(213) |
| 05-May-2001 | Dries Scholtz | Bulls | South Africa | 28-Sep-1979 | 21(220) |
| 17-Mar-2001 | Eduard Coetzee | Sharks | South Africa | 08-Aug-1979 | 21(222) |
| 23-Feb-2001 | Butch James | Sharks | South Africa | 08-Jul-1979 | 21(231) |
| 13-Apr-2001 | James Arlidge | Blues | Japan | 11-Aug-1979 | 21(246) |
| 07-Apr-2001 | Craig Newby | Blues | New Zealand | 27-Jul-1979 | 21(255) |
| 23-Feb-2001 | Ben Blair | Crusaders | New Zealand | 26-Mar-1979 | 21(335) |
| 14-Apr-2001 | Marco Wentzel | Bulls | South Africa | 06-May-1979 | 21(344) |
| 23-Feb-2001 | Friedrich Lombard | Bulls | South Africa | 04-Mar-1979 | 21(357) |
| 24-Feb-2001 | Wylie Human | Lions | South Africa | 26-Feb-1979 | 21(364) |
| 24-Feb-2001 | Deacon Manu | Chiefs | Fiji | 18-Feb-1979 | 22(7) |
| 23-Feb-2001 | David Pusey | Brumbies | Australia | 28-Jan-1979 | 22(27) |
| 22-Apr-2001 | Herkie Kruger | Sharks | South Africa | 21-Mar-1979 | 22(33) |
| 03-Mar-2001 | David Pusey | Brumbies | Australia | 28-Jan-1979 | 22(35) |
| 03-Mar-2001 | David Croft | Queensland Reds | Australia | 22-Jan-1979 | 22(41) |
| 24-Feb-2001 | Matt Dunning | New South Wales | Australia | 19-Dec-1978 | 22(68) |
| 13-Apr-2001 | Justin Wilson | Blues | New Zealand | 25-Dec-1978 | 22(110) |
| 03-Mar-2001 | Frikkie Welsh | Bulls | South Africa | 26-Oct-1978 | 22(129) |
| 14-Apr-2001 | Neil de Koch | Western Stormers | South Africa | 20-Nov-1978 | 22(146) |
| 23-Feb-2001 | Doug Howlett | Blues | New Zealand | 21-Sep-1978 | 22(156) |
| 23-Feb-2001 | Chris Jack | Crusaders | New Zealand | 05-Sep-1978 | 22(172) |
| 10-Mar-2001 | Andrew Hore | Crusaders | New Zealand | 13-Sep-1978 | 22(179) |
| 24-Feb-2001 | David Hill | Chiefs | New Zealand | 31-Jul-1978 | 22(209) |
| 24-Feb-2001 | De Wet Barry | Western Stormers | South Africa | 24-Jun-1978 | 22(246) |
| 03-Mar-2001 | Paul Tito | Hurricanes | New Zealand | 09-Jun-1978 | 22(268) |
| 09-Mar-2001 | Stuart Abbott | Western Stormers | England | 03-Jun-1978 | 22(280) |
| 24-Feb-2001 | Brendan Williams | New South Wales | Australia | 21-May-1978 | 22(280) |
| 10-May-2001 | Tony Penn | Hurricanes | New Zealand | 11-Jul-1978 | 22(304) |
| 23-Feb-2001 | Samiu Vahafolau | Highlanders | Tonga | 24-Apr-1978 | 22(306) |
| 24-Mar-2001 | Drew Hickey | New South Wales | Australia | 16-May-1978 | 22(313) |
| 23-Feb-2001 | John Smit | Sharks | South Africa | 03-Apr-1978 | 22(327) |
| 03-Mar-2001 | Brent Thompson | Hurricanes | New Zealand | 08-Apr-1978 | 22(330) |
| 23-Feb-2001 | Rico Gear | Crusaders | New Zealand | 20-Feb-1978 | 23(3) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).