| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 05-May-2000 |  Franco Mostert | Lions | South Africa | 27-Nov-1990 | 9(160) |
| 25-Feb-2000 | Jerry Collins | Blues | New Zealand | 04-Nov-1980 | 19(112) |
| 05-May-2000 | Aaron Mauger | Crusaders | New Zealand | 29-Nov-1980 | 19(157) |
| 06-May-2000 | George Smith | Brumbies | Australia | 17-Jul-1980 | 19(293) |
| 05-May-2000 | David Lyons | New South Wales | Australia | 16-Jun-1980 | 19(323) |
| 25-Feb-2000 | Orene Ai'I | Blues | New Zealand | 23-Nov-1979 | 20(94) |
| 26-Feb-2000 | Phil Waugh | New South Wales | Australia | 22-Sep-1979 | 20(157) |
| 25-Feb-2000 | Doug Howlett | Blues | New Zealand | 21-Sep-1978 | 21(157) |
| 06-May-2000 | Frikkie Welsh | Bulls | South Africa | 26-Oct-1978 | 21(193) |
| 07-May-2000 | Kuramate Tuirirangi | Hurricanes | New Zealand | 15-Sep-1978 | 21(235) |
| 20-May-2000 | Chris Jack | Crusaders | New Zealand | 05-Sep-1978 | 21(258) |
| 07-May-2000 | De Wet Barry | Western Stormers | South Africa | 24-Jun-1978 | 21(318) |
| 07-May-2000 | Paul Tito | Hurricanes | New Zealand | 09-Jun-1978 | 21(333) |
| 26-Feb-2000 | Nathan Sharpe | Queensland Reds | Australia | 26-Feb-1978 | 21(365) |
| 20-May-2000 | Ben Hurst | Crusaders | New Zealand | 21-Apr-1978 | 22(30) |
| 06-May-2000 | John Smit | Sharks | South Africa | 03-Apr-1978 | 22(34) |
| 27-Feb-2000 | Leon MacDonald | Crusaders | New Zealand | 21-Dec-1977 | 22(68) |
| 26-Feb-2000 | Scott Staniforth | New South Wales | Australia | 12-Dec-1977 | 22(76) |
| 27-Feb-2000 | Greg Somerville | Crusaders | New Zealand | 28-Nov-1977 | 22(91) |
| 27-Feb-2000 | Caleb Ralph | Crusaders | New Zealand | 10-Sep-1977 | 22(170) |
| 26-Feb-2000 | Shane Drahm | Queensland Reds | England | 28-Aug-1977 | 22(182) |
| 25-Feb-2000 | Xavier Rush | Blues | New Zealand | 13-Jul-1977 | 22(227) |
| 27-Feb-2000 | Siaki Tukino | Chiefs | New Zealand | 11-Jul-1977 | 22(231) |
| 25-Feb-2000 | Mark Bartholomeusz | Brumbies | Australia | 30-Jun-1977 | 22(240) |
| 27-Feb-2000 | Mark Ranby | Chiefs | New Zealand | 01-Jun-1977 | 22(271) |
| 25-Feb-2000 | Stirling Mortlock | Brumbies | Australia | 20-May-1977 | 22(281) |
| 26-Feb-2000 | Elton Flatley | Queensland Reds | Australia | 07-May-1977 | 22(295) |
| 26-Feb-2000 | Sean Hardman | Queensland Reds | Australia | 06-May-1977 | 22(296) |
| 26-Feb-2000 | Manuel Edmonds | New South Wales | Australia | 12-Apr-1977 | 22(320) |
| 25-Feb-2000 | Jeremy Paul | Brumbies | Australia | 14-Mar-1977 | 22(348) |
| 26-Feb-2000 | Marc Stcherbina | New South Wales | Australia | 07-Dec-1976 | 23(80) |
| 26-Feb-2000 | Byron Kelleher | Highlanders | New Zealand | 03-Dec-1976 | 23(84) |
| 25-Feb-2000 | Troy Flavell | Blues | New Zealand | 04-Nov-1976 | 23(112) |
| 05-May-2000 | Steve Devine | Blues | New Zealand | 21-Dec-1976 | 23(135) |
| 27-Feb-2000 | Ron Cribb | Crusaders | New Zealand | 07-Jul-1976 | 23(234) |
| 26-Feb-2000 | Romi Ropati | Highlanders | Samoa | 20-Jun-1976 | 23(250) |
| 06-May-2000 | Radike Samo | Brumbies | Australia | 09-Jul-1976 | 23(301) |
| 26-Feb-2000 | Breyton Paulse | Western Stormers | South Africa | 25-Apr-1976 | 23(306) |
| 25-Feb-2000 | Trevor Halstead | Sharks | South Africa | 17-Apr-1976 | 23(313) |
| 26-Feb-2000 | Hendrik Gerber | Western Stormers | South Africa | 12-Apr-1976 | 23(319) |
| 26-Feb-2000 | Nathan Williams | Queensland Reds | Australia | 05-Apr-1976 | 23(326) |
| 25-Feb-2000 | Gaffie Du Toit | Sharks | South Africa | 24-Mar-1976 | 23(337) |
| 26-Feb-2000 | Sam Cordingley | Queensland Reds | Australia | 20-Mar-1976 | 23(342) |
| 27-Feb-2000 | Loki Crichton | Chiefs | Samoa | 14-Mar-1976 | 23(349) |
| 26-Feb-2000 | Pita Alatini | Highlanders | New Zealand | 11-Mar-1976 | 23(351) |
| 27-Feb-2000 | Norm Maxwell | Crusaders | New Zealand | 05-Mar-1976 | 23(358) |
| 26-Feb-2000 | Hottie Louw | Western Stormers | South Africa | 02-Mar-1976 | 23(360) |
| 05-May-2000 | Grant Esterhuizen | Lions | South Africa | 28-Apr-1976 | 24(7) |
| 25-Feb-2000 | Christian Cullen | Hurricanes | New Zealand | 12-Feb-1976 | 24(13) |
| 05-May-2000 | Jannes Labuschagne | Lions | South Africa | 16-Apr-1976 | 24(19) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).