| Penalties | Club | VS Club | Date | |
|---|---|---|---|---|
| 8 | Orrell | Moseley | 16-09-2000 | Match details |
| 7 | Leeds Tykes | Manchester | 07-10-2000 | Match details |
| 7 | Birmingham Solihull | Coventry | 30-09-2000 | Match details |
| 7 | Exeter Chiefs | Wakefield | 16-09-2000 | Match details |
| 7 | Henley Hawks | London Welsh | 18-11-2000 | Match details |
| 7 | Worcester Warriors | Moseley | 09-12-2000 | Match details |
| 6 | Manchester | Otley | 25-11-2000 | Match details |
| 6 | Coventry | Birmingham Solihull | 30-09-2000 | Match details |
| 6 | Moseley | Coventry | 18-02-2001 | Match details |
| 6 | Waterloo | Exeter Chiefs | 23-09-2000 | Match details |
| 6 | London Welsh | Worcester Warriors | 25-11-2000 | Match details |
| 5 | Bedford Blues | Henley Hawks | 09-09-2000 | Match details |
| 5 | Otley | Worcester Warriors | 19-11-2000 | Match details |
| 5 | Wakefield | Exeter Chiefs | 16-09-2000 | Match details |
