| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 12-Jun-2010 | Simon Shaw | England | 01-Sep-1973 | 36(284) |
| 21-Nov-2009 | Duncan Bell | England | 01-Oct-1974 | 35(52) |
| 21-Nov-2009 | Joe Worsley | England | 14-Jun-1977 | 32(160) |
| 19-Jun-2010 | Lewis Moody | England | 12-Jun-1978 | 32(7) |
| 19-Jun-2010 | Steve Thompson | England | 15-Jul-1978 | 31(339) |
| 19-Jun-2010 | Nick Easter | England | 15-Aug-1978 | 31(308) |
| 19-Jun-2010 | Mike Tindall | England | 18-Oct-1978 | 31(244) |
| 19-Jun-2010 | Tom Palmer | England | 27-Mar-1979 | 31(84) |
| 19-Jun-2010 | Tim Payne | England | 29-Apr-1979 | 31(51) |
| 21-Nov-2009 | Jonny Wilkinson | England | 25-May-1979 | 30(181) |
| 19-Jun-2010 | Mark Cueto | England | 26-Dec-1979 | 30(176) |
| 21-Nov-2009 | Steve Borthwick | England | 12-Oct-1979 | 30(41) |
| 21-Nov-2009 | Ayoola Erinle | England | 20-Feb-1980 | 29(275) |
| 14-Nov-2009 | Louis Deacon | England | 07-Oct-1980 | 29(38) |
| 19-Jun-2010 | Shontayne Hape | England | 30-Jan-1982 | 28(140) |
| 21-Nov-2009 | Paul Hodgson | England | 25-Apr-1982 | 27(210) |
| 21-Nov-2009 | Dan Hipkiss | England | 04-Jun-1982 | 27(170) |
| 21-Nov-2009 | Ugo Monye | England | 13-Apr-1983 | 26(223) |
| 19-Jun-2010 | Ben Foden | England | 22-Jul-1985 | 24(332) |
| 19-Jun-2010 | Toby Flood | England | 08-Aug-1985 | 24(315) |
| 21-Nov-2009 | James Haskell | England | 02-Apr-1985 | 24(233) |
| 19-Jun-2010 | Tom Croft | England | 07-Nov-1985 | 24(224) |
| 07-Nov-2009 | Dave Wilson | England | 09-Apr-1985 | 24(212) |
| 21-Nov-2009 | Dylan Hartley | England | 24-Mar-1986 | 23(242) |
| 12-Jun-2010 | Danny Care | England | 02-Jan-1987 | 23(161) |
| 07-Nov-2009 | Jordan Crane | England | 03-Jun-1986 | 23(157) |
| 14-Nov-2009 | Shane Geraghty | England | 12-Aug-1986 | 23(94) |
| 19-Jun-2010 | Chris Ashton | England | 29-Mar-1987 | 23(82) |
| 19-Jun-2010 | Dan Cole | England | 09-May-1987 | 23(41) |
| 21-Nov-2009 | Matt Banahan | England | 30-Dec-1986 | 22(327) |
| 19-Jun-2010 | Courtney Lawes | England | 23-Feb-1989 | 21(116) |
| 19-Jun-2010 | Ben Youngs | England | 05-Sep-1989 | 20(287) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).