| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 06-Mar-2021 | Manu Vunipola | England | 04-May-2000 | 20(306) |
| 06-Mar-2021 | Kapeliele Pifeletijr | United States | 01-Sep-1999 | 21(187) |
| 11-Apr-2021 | Joel Kpoku | England | 22-Jun-1999 | 21(294) |
| 11-Apr-2021 | Andrew Christie | Scotland | 22-Mar-1999 | 22(21) |
| 06-Mar-2021 | Sean Reffell | England | 04-Nov-1998 | 22(123) |
| 06-Mar-2021 | Elliot Obatynibo | England | 09-Oct-1998 | 22(149) |
| 17-May-2021 | Alsitair Crossdale | England | 09-Jan-1998 | 23(128) |
| 28-Mar-2021 | Dom Morris | England | 29-Jul-1997 | 23(242) |
| 06-Mar-2021 | Callum Hunter-Hills | Scotland | 27-Feb-1997 | 24(7) |
| 13-Mar-2021 | Rotimi Segun | England | 28-Dec-1996 | 24(75) |
| 01-May-2021 | Tom Whiteley | England | 17-Dec-1995 | 25(136) |
| 18-Apr-2021 | Nick Tompkins | Wales | 16-Feb-1995 | 26(62) |
| 11-Apr-2021 | Maro Itoje | England | 28-Oct-1994 | 26(166) |
| 01-May-2021 | Janco Venter | Namibia | 19-Sep-1994 | 26(225) |
| 01-May-2021 | Tom Woolstencroft | England | 19-Sep-1994 | 26(225) |
| 01-May-2021 | Alec Clarey | England | 08-Feb-1994 | 27(82) |
| 28-Mar-2021 | William Hooley | United States | 28-Nov-1993 | 27(120) |
| 11-Apr-2021 | Billy Vunipola | England | 03-Nov-1992 | 28(159) |
| 11-Apr-2021 | Elliot Daly | England | 08-Oct-1992 | 28(185) |
| 06-Mar-2021 | Aled Davies | Wales | 19-Jul-1992 | 28(230) |
| 06-Mar-2021 | Alex Lewington | England | 20-Sep-1991 | 29(168) |
| 18-Apr-2021 | Owen Farrell | England | 21-Sep-1991 | 29(210) |
| 11-Apr-2021 | Makovina Vunipola | England | 13-Jan-1991 | 30(89) |
| 06-Mar-2021 | Jackson Wray | England | 10-Nov-1990 | 30(117) |
| 28-Mar-2021 | Jamie George | England | 20-Oct-1990 | 30(160) |
| 06-Mar-2021 | Vincent Koch | South Africa | 13-Mar-1990 | 30(359) |
| 06-Mar-2021 | Richard Barrington | England | 24-Sep-1989 | 31(163) |
| 06-Mar-2021 | Duncan Taylor | Scotland | 05-Sep-1989 | 31(183) |
| 17-May-2021 | Calum Clark | England | 10-Jun-1989 | 31(342) |
| 06-Mar-2021 | Sean Maitland | Scotland | 14-Sep-1988 | 32(173) |
| 06-Mar-2021 | Juan Pablo Socino | Argentina | 30-May-1988 | 32(280) |
| 22-May-2021 | Alex Goode | England | 07-May-1988 | 33(15) |
| 06-Mar-2021 | Michael Rhodes | South Africa | 19-Dec-1987 | 33(78) |
| 06-Mar-2021 | Tim Swinson | Scotland | 11-Feb-1987 | 34(24) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).