| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 03-Jul-2021 |  Jordan Hendrikse | Lions | South Africa | 28-Jun-2002 | 19(5) |
| 03-Jul-2021 | Louis Rees-Zammitt | British Lions (NT) | Wales | 02-Feb-2001 | 20(151) |
| 03-Jul-2021 | Sibusio Sangweni | Lions | South Africa | 27-Nov-2000 | 20(218) |
| 10-Jul-2021 | Celimpilo Gumede | Sharks | South Africa | 08-Jul-2000 | 21(2) |
| 07-Jul-2021 | Jaden Hendrikse | Sharks | South Africa | 23-Mar-2000 | 21(106) |
| 17-Jul-2021 | Evan Roos | Western Stormers | South Africa | 21-Jan-2000 | 21(178) |
| 07-Jul-2021 | Thaakir Abrahams | Sharks | South Africa | 10-Jan-2000 | 21(179) |
| 07-Jul-2021 | Fezokuhle Mbatha | Sharks | South Africa | 02-Aug-1999 | 21(340) |
| 10-Jul-2021 | Marnus Potgieter | Sharks | South Africa | 20-Jun-1999 | 22(21) |
| 07-Jul-2021 | Phendulani Buthelezi | Sharks | South Africa | 30-May-1999 | 22(39) |
| 03-Jul-2021 | Francke Horn | Lions | South Africa | 24-May-1999 | 22(41) |
| 10-Jul-2021 | Ntuthuko Mchunu | Sharks | South Africa | 05-Apr-1999 | 22(97) |
| 10-Jul-2021 | Murray Koster | Sharks | South Africa | 12-Feb-1999 | 22(149) |
| 17-Jul-2021 | Marcus Smith | British Lions (NT) | England | 14-Feb-1999 | 22(154) |
| 10-Jul-2021 | Dylan Richardson | Sharks | Scotland | 15-Jan-1999 | 22(177) |
| 26-Jun-2021 |  Siosaia Fifita | Japan | Japan | 20-Dec-1998 | 22(189) |
| 17-Jul-2021 | Leon Lyons | Western Stormers | South Africa | 02-Dec-1998 | 22(228) |
| 03-Jul-2021 | Vincent Tshituka | Lions | South Africa | 10-Sep-1998 | 22(297) |
| 07-Jul-2021 | Tom Curry | British Lions (NT) | England | 15-Jun-1998 | 23(22) |
| 03-Jul-2021 | Nathan McBeth | Lions | South Africa | 08-Jun-1998 | 23(25) |
| 03-Jul-2021 | Manuel Rass | Lions | South Africa | 19-Apr-1998 | 23(75) |
| 03-Jul-2021 | PJ Botha | Lions | South Africa | 20-Jan-1998 | 23(164) |
| 07-Jul-2021 | Ruben van Heerden | Sharks | South Africa | 27-Oct-1997 | 23(253) |
| 03-Jul-2021 | Rainhard Nothnagel | Lions | South Africa | 25-Sep-1997 | 23(281) |
| 17-Jul-2021 | Ernst van Rhyn | Western Stormers | South Africa | 19-Sep-1997 | 23(301) |
| 17-Jul-2021 | Nama Xaba | Western Stormers | South Africa | 26-Jul-1997 | 23(356) |
| 07-Jul-2021 | Immanuel Libbok | Sharks | South Africa | 15-Jul-1997 | 23(357) |
| 07-Jul-2021 | Khuthuzani Mchunu | Sharks | South Africa | 01-Jul-1997 | 24(6) |
| 07-Jul-2021 | Curwin Bosch | Sharks | South Africa | 25-Jun-1997 | 24(12) |
| 07-Jul-2021 | James Venter | Sharks | South Africa | 21-Aug-1996 | 24(320) |
| 17-Jul-2021 | Edwil van der Merwe | Western Stormers | South Africa | 12-Apr-1996 | 25(96) |
| 03-Jul-2021 | Ruben Schoeman | Lions | South Africa | 14-Mar-1996 | 25(111) |
| 07-Jul-2021 | Zander Fagerson | British Lions (NT) | Scotland | 19-Jan-1996 | 25(170) |
| 07-Jul-2021 | Jeremy Ward | Sharks | South Africa | 10-Jan-1996 | 25(179) |
| 07-Jul-2021 | Adam Beard | British Lions (NT) | Wales | 07-Jan-1996 | 25(182) |
| 07-Jul-2021 | Marius Louw | Sharks | South Africa | 24-Oct-1995 | 25(257) |
| 17-Jul-2021 | Leolin Zas | Western Stormers | South Africa | 20-Oct-1995 | 25(271) |
| 31-Jul-2021 |  Jasper Wiese | South Africa | South Africa | 21-Oct-1995 | 25(284) |
| 17-Jul-2021 | Johan du Toit | Western Stormers | South Africa | 08-Sep-1995 | 25(313) |
| 03-Jul-2021 | Sibahle Maxwane | Lions | South Africa | 14-Aug-1995 | 25(324) |
| 07-Jul-2021 | Hyron Andrews | Sharks | South Africa | 06-Jul-1995 | 26(2) |
| 24-Jul-2021 | Ox Nche | South Africa | South Africa | 23-Jul-1995 | 26(2) |
| 26-Jun-2021 | Duhan van der Merwe | British Lions | Scotland | 04-Jun-1995 | 26(23) |
| 10-Jul-2021 | Kerron van Vuuren | Sharks | South Africa | 23-May-1995 | 26(49) |
| 03-Jul-2021 | EW Viljoen | Lions | South Africa | 09-May-1995 | 26(56) |
| 26-Jun-2021 | Josh Adams | British Lions | Wales | 21-Apr-1995 | 26(67) |
| 17-Jul-2021 | JD Schickerling | Western Stormers | South Africa | 09-May-1995 | 26(70) |
| 17-Jul-2021 | Daniel du Plessis | Western Stormers | South Africa | 17-Mar-1995 | 26(123) |
| 10-Jul-2021 | Le Roux Roets | Sharks | South Africa | 14-Jan-1995 | 26(178) |
| 07-Jul-2021 | Sam Simmonds | British Lions (NT) | England | 10-Nov-1994 | 26(240) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).