| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 15-May-2021 |  Juan Manuel Alonso | Penarol | Uruguay | 19-Sep-2001 | 19(238) |
| 08-May-2021 | Martin Vaca | Jaguares XV | Argentina | 02-Jun-2001 | 19(340) |
| 08-May-2021 | Jeronimo Garcia Vara | Olimpia Lions | Argentina | 10-Jan-2001 | 20(118) |
| 08-May-2021 | Juan Martin Gonzalez Samso | Jaguares XV | Argentina | 14-Nov-2000 | 20(175) |
| 08-May-2021 | Matias Garafulic | Selknam | Chile | 01-Sep-2000 | 20(249) |
| 08-May-2021 | Felipe Aliaga | Penarol | Uruguay | 14-Sep-1999 | 21(237) |
| 08-May-2021 | Jose Iruleguy | Penarol | Uruguay | 23-Jun-1999 | 21(320) |
| 08-May-2021 | Tomas Acosta Pimentel | Olimpia Lions | Argentina | 01-Jun-1999 | 21(342) |
| 15-May-2021 | Beltazar Amaya | Penarol | Uruguay | 26-May-1999 | 21(355) |
| 08-May-2021 | Juan Pablo Castro | Jaguares XV | Argentina | 20-Apr-1999 | 22(19) |
| 08-May-2021 | Ignacio Gandini | Olimpia Lions | Argentina | 17-Mar-1999 | 22(53) |
| 08-May-2021 | Francisco Minervino | Jaguares XV | Argentina | 05-Feb-1999 | 22(93) |
| 08-May-2021 | Manuel Nogues | Penarol | Argentina | 27-Dec-1998 | 22(133) |
| 08-May-2021 | Leopoldo Herrera | Olimpia Lions | Argentina | 12-Dec-1998 | 22(148) |
| 08-May-2021 | Nicolas Garafulic | Selknam | Chile | 11-Sep-1998 | 22(240) |
| 08-May-2021 | Manuel Ardao | Penarol | Uruguay | 09-Sep-1998 | 22(242) |
| 08-May-2021 | Rodrigo Martinez | Olimpia Lions | Argentina | 07-Jul-1998 | 22(306) |
| 08-May-2021 | Juan Bautista Daireaux | Jaguares XV | Argentina | 23-Jun-1998 | 22(320) |
| 08-May-2021 | Agustin Segura | Jaguares XV | Argentina | 21-Mar-1998 | 23(48) |
| 08-May-2021 | Santiago Civetta | Penarol | Uruguay | 28-Feb-1998 | 23(69) |
| 08-May-2021 | Tomas Cubilla | Jaguares XV | Argentina | 07-Feb-1998 | 23(90) |
| 08-May-2021 | Santiago Videla | Selknam | Chile | 16-Jan-1998 | 23(112) |
| 08-May-2021 | Clemente Saavedra | Selknam | Chile | 15-Dec-1997 | 23(144) |
| 08-May-2021 | Domingo Saavedra | Selknam | Chile | 15-Dec-1997 | 23(144) |
| 08-May-2021 | Martin Roger | Penarol | Argentina | 22-Oct-1997 | 23(198) |
| 08-May-2021 | Tomas Albornoz | Jaguares XV | Argentina | 17-Sep-1997 | 23(233) |
| 08-May-2021 | Franco Molina | Jaguares XV | Argentina | 28-Aug-1997 | 23(253) |
| 08-May-2021 | Javier Carrasco | Selknam | Chile | 24-Aug-1997 | 23(257) |
| 08-May-2021 | Alfonso Escobar | Selknam | Chile | 17-Aug-1997 | 23(264) |
| 08-May-2021 | Ignacio Inchauspe | Olimpia Lions | Argentina | 14-Jul-1997 | 23(298) |
| 08-May-2021 | Nahuel Milan | Penarol | Argentina | 30-Jun-1997 | 23(312) |
| 08-May-2021 | Augusto Bohme | Selknam | Chile | 11-Jun-1997 | 23(331) |
| 08-May-2021 | Guillermo Pujadas | Penarol | Uruguay | 06-Feb-1997 | 24(91) |
| 08-May-2021 | Lucas Favre | Olimpia Lions | Argentina | 20-Nov-1996 | 24(169) |
| 08-May-2021 | Tomas Inciarte | Penarol | Uruguay | 22-Oct-1996 | 24(198) |
| 08-May-2021 | Martin Sigren | Selknam | Chile | 14-May-1996 | 24(359) |
| 08-May-2021 | Patricio Baronio-Gauna | Selknam | Argentina | 06-May-1996 | 25(2) |
| 08-May-2021 | Conrado Roura | Penarol | Argentina | 28-Feb-1996 | 25(70) |
| 08-May-2021 | Rodrigo Fernandez | Selknam | Chile | 08-Feb-1996 | 25(90) |
| 08-May-2021 | Carlos Repetto | Olimpia Lions | Argentina | 08-Jan-1996 | 25(121) |
| 15-May-2021 | Federico Wegrzyn | Jaguares XV | Argentina | 08-Jan-1996 | 25(128) |
| 08-May-2021 | Santiago Portillo | Selknam | Argentina | 21-Nov-1995 | 25(169) |
| 08-May-2021 | Julio Blanc | Selknam | Chile | 06-Jul-1995 | 25(307) |
| 08-May-2021 | Francisco Gorrissen | Jaguares XV | Argentina | 30-Aug-1994 | 26(252) |
| 08-May-2021 | Lucas Santa Cruz | Olimpia Lions | Argentina | 23-Apr-1994 | 27(15) |
| 08-May-2021 | Lautaro Bavaro | Jaguares XV | Argentina | 09-Apr-1994 | 27(29) |
| 15-May-2021 | Sebastian Cancelliere | Jaguares XV | Argentina | 17-Sep-1993 | 27(240) |
| 08-May-2021 | Matias Dittus | Selknam | Chile | 16-Jul-1993 | 27(296) |
| 08-May-2021 | Nicolas Freitas | Penarol | Uruguay | 03-Jul-1993 | 27(309) |
| 08-May-2021 | Sebastian Urbieta | Olimpia Lions | Paraguay | 11-May-1993 | 27(362) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).