| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 02-May-2021 |  Pedro Rubiolo | Jaguares XV | Argentina | 12-Dec-2002 | 18(142) |
| 25-Mar-2021 | Santiago Alvarez | Penarol | Uruguay | 24-Dec-2001 | 19(91) |
| 21-Mar-2021 | Juan Manuel Alonso | Penarol | Uruguay | 19-Sep-2001 | 19(183) |
| 11-Apr-2021 | Beltran Salese | Jaguares XV | Argentina | 06-Sep-2001 | 19(217) |
| 21-Mar-2021 | Joaquin Oviedo | Jaguares XV | Argentina | 17-Jul-2001 | 19(247) |
| 21-Mar-2021 | Carlos Deus | Penarol | Uruguay | 05-Jul-2001 | 19(259) |
| 21-Mar-2021 | Martin Vaca | Jaguares XV | Argentina | 02-Jun-2001 | 19(292) |
| 25-Mar-2021 | Adrio Melo | Corinthians | Brazil | 21-Mar-2001 | 20(4) |
| 21-Apr-2021 | Lucas Bianchi | Penarol | Uruguay | 26-Mar-2001 | 20(26) |
| 16-Mar-2021 | Jeronimo Garcia Vara | Olimpia Lions | Argentina | 10-Jan-2001 | 20(65) |
| 16-Apr-2021 | Ignacio Ruiz | Jaguares XV | Argentina | 03-Jan-2001 | 20(103) |
| 21-Mar-2021 | Santiago Pedrero | Selknam | Argentina | 30-Nov-2000 | 20(111) |
| 21-Mar-2021 | Juan Martin Gonzalez Samso | Jaguares XV | Argentina | 14-Nov-2000 | 20(127) |
| 04-Apr-2021 | Mateo Perillo | Penarol | Uruguay | 11-Nov-2000 | 20(144) |
| 16-Mar-2021 | Felipe Puertas | Cafeteros Pro | Argentina | 19-Sep-2000 | 20(178) |
| 16-Mar-2021 | Matias Garafulic | Selknam | Chile | 01-Sep-2000 | 20(196) |
| 21-Mar-2021 | Manuel Bernstein | Corinthians | Argentina | 18-May-2000 | 20(307) |
| 21-Mar-2021 | Felipe Arcos Perez | Penarol | Uruguay | 17-May-2000 | 20(308) |
| 11-Apr-2021 | Tomas Bernasconi | Jaguares XV | Argentina | 26-May-2000 | 20(320) |
| 21-Mar-2021 | Ramiro Tallone Naddaf | Cafeteros Pro | Argentina | 18-Apr-2000 | 20(337) |
| 16-Apr-2021 | Rafael Iriarte | Jaguares XV | Argentina | 24-Mar-2000 | 21(23) |
| 16-Mar-2021 | Nicolas Roger Farias | Cafeteros Pro | Argentina | 11-Jan-2000 | 21(65) |
| 16-Apr-2021 | Mariano Muntaner | Olimpia Lions | Argentina | 05-Feb-2000 | 21(71) |
| 16-Mar-2021 | Raimundo Martinez | Selknam | Chile | 25-Nov-1999 | 21(112) |
| 25-Mar-2021 | Felipe Cunha | Corinthians | Brazil | 01-Dec-1999 | 21(115) |
| 16-Mar-2021 | Felipe Aliaga | Penarol | Uruguay | 14-Sep-1999 | 21(184) |
| 11-Apr-2021 | Geronimo Priscantelli | Jaguares XV | Argentina | 23-Aug-1999 | 21(232) |
| 16-Mar-2021 | Facundo Pueyrredon | Cafeteros Pro | Argentina | 23-Jul-1999 | 21(237) |
| 16-Apr-2021 | Alvaro Gamarra | Cafeteros Pro | Colombia | 15-Aug-1999 | 21(245) |
| 21-Mar-2021 | Jose Iruleguy | Penarol | Uruguay | 23-Jun-1999 | 21(272) |
| 16-Mar-2021 | Salvador Lues | Selknam | Chile | 11-Jun-1999 | 21(279) |
| 21-Mar-2021 | Beltazar Amaya | Penarol | Uruguay | 26-May-1999 | 21(300) |
| 25-Mar-2021 | Joaquin Pellandini | Olimpia Lions | Argentina | 27-May-1999 | 21(303) |
| 16-Apr-2021 | Gabriel Quirino | Corinthians | Brazil | 07-Jun-1999 | 21(314) |
| 27-Apr-2021 | Tomas Acosta Pimentel | Olimpia Lions | Argentina | 01-Jun-1999 | 21(331) |
| 25-Mar-2021 | Juan Pablo Castro | Jaguares XV | Argentina | 20-Apr-1999 | 21(340) |
| 21-Mar-2021 | Santiago Chocobares | Jaguares XV | Argentina | 31-Mar-1999 | 21(356) |
| 16-Mar-2021 | Ignacio Gandini | Olimpia Lions | Argentina | 17-Mar-1999 | 21(365) |
| 25-Mar-2021 | Ezequiel Ramos | Penarol | Uruguay | 24-Mar-1999 | 22(2) |
| 16-Mar-2021 | Gonzalo Garcia | Cafeteros Pro | Argentina | 05-Mar-1999 | 22(12) |
| 21-Mar-2021 | Francisco Minervino | Jaguares XV | Argentina | 05-Feb-1999 | 22(45) |
| 16-Mar-2021 | Juanjuan Garese | Penarol | Uruguay | 25-Jan-1999 | 22(51) |
| 26-Apr-2021 | Ignacio Peculo | Penarol | Uruguay | 22-Feb-1999 | 22(64) |
| 16-Mar-2021 | Manuel Nogues | Penarol | Argentina | 27-Dec-1998 | 22(80) |
| 16-Mar-2021 | Leopoldo Herrera | Olimpia Lions | Argentina | 12-Dec-1998 | 22(95) |
| 25-Mar-2021 | Mateo Vinals | Penarol | Uruguay | 07-Oct-1998 | 22(170) |
| 16-Mar-2021 | Alejandro Navarro | Cafeteros Pro | Colombia | 14-Sep-1998 | 22(184) |
| 16-Mar-2021 | Nicolas Garafulic | Selknam | Chile | 11-Sep-1998 | 22(187) |
| 16-Mar-2021 | Manuel Ardao | Penarol | Uruguay | 09-Sep-1998 | 22(189) |
| 16-Mar-2021 | Rodrigo Martinez | Olimpia Lions | Argentina | 07-Jul-1998 | 22(253) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).