| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 02-Apr-2021 |  Jeremy Williams | New South Wales | Australia | 02-Dec-2000 | 20(121) |
| 19-Feb-2021 | Gus Bell | New South Wales | Australia | 04-Oct-2000 | 20(138) |
| 27-Feb-2021 | Mark Nawaqanitawase | New South Wales | Australia | 11-Sep-2000 | 20(169) |
| 19-Feb-2021 | Will Harris | New South Wales | Australia | 08-Jun-2000 | 20(256) |
| 19-Feb-2021 | Joey Walton | New South Wales | Australia | 27-May-2000 | 20(268) |
| 19-Feb-2021 | Byron Ralston | Western Force | Australia | 03-May-2000 | 20(292) |
| 19-Feb-2021 | Jordan Petaia | Queensland Reds | Australia | 14-Mar-2000 | 20(342) |
| 19-Feb-2021 | Carlo Tizzano | New South Wales | Australia | 02-Feb-2000 | 21(18) |
| 19-Feb-2021 | Noah Lolesio | Brumbies | Australia | 18-Dec-1999 | 21(64) |
| 19-Feb-2021 | Harry Wilson | Queensland Reds | Australia | 22-Nov-1999 | 21(90) |
| 02-Apr-2021 | Max Douglas | New South Wales | Australia | 01-Jan-2000 | 21(92) |
| 26-Feb-2021 | Trevor Hosea | Melbourne Rebels | Australia | 24-Nov-1999 | 21(95) |
| 02-Apr-2021 | Nick Frost | Brumbies | Australia | 10-Oct-1999 | 21(175) |
| 20-Mar-2021 | Ilaisa Droasese | Queensland Reds | Fiji | 13-Sep-1999 | 21(189) |
| 19-Feb-2021 | Will Harrison | New South Wales | Australia | 30-Jul-1999 | 21(205) |
| 08-May-2021 | Lachlan Lonergan | Brumbies | Australia | 11-Oct-1999 | 21(210) |
| 19-Feb-2021 | Fraser McReight | Queensland Reds | Australia | 19-Feb-1999 | 22(1) |
| 17-Apr-2021 | Ben Donaldson | New South Wales | Australia | 04-Apr-1999 | 22(14) |
| 06-Mar-2021 | Josh Kemeny | Melbourne Rebels | Australia | 29-Nov-1998 | 22(98) |
| 19-Feb-2021 | Len Ikitau | Brumbies | Australia | 01-Oct-1998 | 22(142) |
| 19-Feb-2021 | Tata McDermott | Queensland Reds | Australia | 18-Sep-1998 | 22(155) |
| 19-Feb-2021 | Robert Valetini | Brumbies | Australia | 03-Sep-1998 | 22(170) |
| 26-Feb-2021 | Cabous Eloff | Melbourne Rebels | South Africa | 30-Jul-1998 | 22(212) |
| 19-Feb-2021 | James Ramm | New South Wales | Australia | 30-Apr-1998 | 22(296) |
| 18-Apr-2021 | Bayley Kuenzle | Brumbies | Australia | 18-Jun-1998 | 22(305) |
| 19-Feb-2021 | Okoumene Paisami | Queensland Reds | Australia | 10-Apr-1998 | 22(316) |
| 13-Mar-2021 | Tom Ross | Brumbies | Australia | 29-Apr-1998 | 22(319) |
| 19-Feb-2021 | Mackenzie Hansen | Brumbies | Ireland | 27-Mar-1998 | 22(330) |
| 19-Feb-2021 | Angus Blyth | Queensland Reds | Australia | 04-Mar-1998 | 22(353) |
| 19-Feb-2021 | Hamish Stewart | Queensland Reds | Australia | 03-Mar-1998 | 22(354) |
| 09-Apr-2021 | Jordan Olowofela | Western Force | England | 07-Apr-1998 | 23(2) |
| 18-Apr-2021 | Ryan Lonergan | Brumbies | Australia | 06-Apr-1998 | 23(12) |
| 23-Apr-2021 | Liam Wright | Queensland Reds | Australia | 06-Nov-1997 | 23(168) |
| 26-Feb-2021 | Lachlan Anderson | Melbourne Rebels | Australia | 27-Aug-1997 | 23(183) |
| 19-Feb-2021 | Darcy Swain | Brumbies | Australia | 05-Jul-1997 | 23(229) |
| 02-Apr-2021 | Tom Wright | Brumbies | Australia | 21-Jul-1997 | 23(255) |
| 12-Mar-2021 | Ryan McCauley | Western Force | Australia | 08-Jun-1997 | 23(277) |
| 19-Feb-2021 | Izaia Perese | New South Wales | Australia | 17-May-1997 | 23(278) |
| 05-Mar-2021 | Tim Anstee | Western Force | Australia | 19-May-1997 | 23(290) |
| 19-Feb-2021 | Angus Scott-Young | Queensland Reds | Australia | 23-Apr-1997 | 23(302) |
| 19-Feb-2021 | Tom Horton | New South Wales | Australia | 18-Apr-1997 | 23(307) |
| 19-Feb-2021 | Sam Caird | New South Wales | New Zealand | 18-Mar-1997 | 23(338) |
| 19-Feb-2021 | Harry Johnson-Holmes | New South Wales | Australia | 02-Mar-1997 | 23(354) |
| 26-Feb-2021 | Pone Fa'amuasili | Melbourne Rebels | Australia | 26-Feb-1997 | 24(0) |
| 19-Feb-2021 | Jack Maddocks | New South Wales | Australia | 05-Feb-1997 | 24(14) |
| 26-Feb-2021 | Jordan Uelese | Melbourne Rebels | Australia | 24-Jan-1997 | 24(33) |
| 09-Apr-2021 | Robert Leota | Melbourne Rebels | New Zealand | 03-Mar-1997 | 24(37) |
| 27-Feb-2021 | Lachlan Swinton | New South Wales | Australia | 16-Jan-1997 | 24(42) |
| 19-Feb-2021 | Seru Uru | Queensland Reds | Australia | 03-Jan-1997 | 24(47) |
| 26-Mar-2021 | Jack McGregor | Western Force | Australia | 10-Jan-1997 | 24(75) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).