| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 09-Feb-2020 |  Arthur Vincent | France | France | 30-Sep-1999 | 20(132) |
| 24-Oct-2020 |  Paolo Garbisi | Italy | Italy | 03-Jun-2000 | 20(143) |
| 02-Feb-2020 | Romain Ntamack | France | France | 01-May-1999 | 20(277) |
| 02-Feb-2020 |  Tom Curry | England | England | 15-Jun-1998 | 21(232) |
| 01-Feb-2020 | Niccolo Cannone | Italy | Italy | 17-May-1998 | 21(260) |
| 01-Feb-2020 |  Caelan Doris | Ireland | Ireland | 02-Apr-1998 | 21(305) |
| 01-Feb-2020 |  Aaron Wainwright | Wales | Wales | 25-Sep-1997 | 22(129) |
| 01-Feb-2020 | Jordan Larmour | Ireland | Ireland | 10-Jun-1997 | 22(236) |
| 31-Oct-2020 | Rhys Carre | Wales | Wales | 08-Feb-1998 | 22(266) |
| 24-Oct-2020 | Danilo Fischetti | Italy | Italy | 26-Jan-1998 | 22(272) |
| 02-Feb-2020 | Gregory Alldritt | France | France | 23-Mar-1997 | 22(316) |
| 01-Feb-2020 |  Blair Kinghorn | Scotland | Scotland | 18-Jan-1997 | 23(13) |
| 01-Feb-2020 | Giosue Zilocchi | Italy | Italy | 15-Jan-1997 | 23(16) |
| 31-Oct-2020 | Shane Lewis-Hughes | Wales | Wales | 20-Sep-1997 | 23(41) |
| 01-Feb-2020 | Scott Cummings | Scotland | Scotland | 03-Dec-1996 | 23(59) |
| 02-Feb-2020 | Antoine Dupont | France | France | 15-Nov-1996 | 23(78) |
| 02-Feb-2020 | George Furbank | England | England | 17-Oct-1996 | 23(107) |
| 01-Feb-2020 | Adam Hastings | Scotland | Scotland | 05-Oct-1996 | 23(118) |
| 31-Oct-2020 | Darcy Graham | Scotland | Scotland | 21-Jun-1997 | 23(132) |
| 08-Mar-2020 | Damian Penaud | France | France | 25-Sep-1996 | 23(164) |
| 01-Feb-2020 | Jamie Ritchie | Scotland | Scotland | 16-Aug-1996 | 23(168) |
| 01-Feb-2020 | James Ryan | Ireland | Ireland | 24-Jul-1996 | 23(191) |
| 02-Feb-2020 | Sam Underhill | England | England | 22-Jul-1996 | 23(194) |
| 01-Feb-2020 | Matteo Minozzi | Italy | Italy | 04-Jun-1996 | 23(241) |
| 01-Feb-2020 | Jacob Stockdale | Ireland | Ireland | 03-Apr-1996 | 23(303) |
| 01-Feb-2020 | Zander Fagerson | Scotland | Scotland | 19-Jan-1996 | 24(13) |
| 31-Oct-2020 | Owen Watkin | Wales | Wales | 12-Oct-1996 | 24(19) |
| 01-Feb-2020 | Dillon Lewis | Wales | Wales | 04-Jan-1996 | 24(28) |
| 08-Feb-2020 | Lewis Ludlam | England | England | 08-Dec-1995 | 24(62) |
| 01-Feb-2020 | Jake Polledri | Italy | Italy | 08-Nov-1995 | 24(85) |
| 24-Oct-2020 | Hugo Keenan | Ireland | Ireland | 18-Jun-1996 | 24(128) |
| 08-Feb-2020 | Magnus Bradbury | Scotland | Scotland | 23-Aug-1995 | 24(169) |
| 24-Oct-2020 | Will Connors | Ireland | Ireland | 04-Apr-1996 | 24(203) |
| 02-Feb-2020 | Charlie Ewels | England | England | 29-Jun-1995 | 24(218) |
| 02-Feb-2020 | Julian Marchand | France | France | 10-May-1995 | 24(268) |
| 24-Oct-2020 | Andrew Porter | Ireland | Ireland | 16-Jan-1996 | 24(282) |
| 01-Feb-2020 | Josh Adams | Wales | Wales | 21-Apr-1995 | 24(286) |
| 08-Feb-2020 | Nick Tompkins | Wales | Wales | 16-Feb-1995 | 24(357) |
| 01-Feb-2020 | Gary Ringrose | Ireland | Ireland | 26-Jan-1995 | 25(6) |
| 01-Feb-2020 | Tomos Williams | Wales | Wales | 01-Jan-1995 | 25(31) |
| 24-Oct-2020 | Marco Lazzaroni | Italy | Italy | 18-Aug-1995 | 25(68) |
| 02-Feb-2020 | Maro Itoje | England | England | 28-Oct-1994 | 25(97) |
| 31-Oct-2020 | James Lang | Scotland | Scotland | 04-Apr-1995 | 25(211) |
| 01-Feb-2020 | Sebastian Negri | Italy | Italy | 30-Jun-1994 | 25(216) |
| 31-Oct-2020 | Ryan Elias | Wales | Wales | 07-Jan-1995 | 25(298) |
| 22-Feb-2020 | Ross Moriarty | Wales | Wales | 18-Apr-1994 | 25(310) |
| 02-Feb-2020 | Gael Fickou | France | France | 26-Mar-1994 | 25(313) |
| 02-Feb-2020 | Francois Cros | France | France | 25-Mar-1994 | 25(314) |
| 01-Feb-2020 | Callum Braley | Italy | Italy | 23-Mar-1994 | 25(315) |
| 01-Feb-2020 | Jonny Gray | Scotland | Scotland | 14-Mar-1994 | 25(324) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).