| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 01-May-2016 |  Taylor Prell | Leeds Tykes | England | 03-Jul-1996 | 19(302) |
| 01-May-2016 | Jack Walker | Leeds Tykes | England | 06-May-1996 | 19(360) |
| 01-May-2016 | George Perkins | Bedford Blues | England | 27-Feb-1996 | 20(64) |
| 01-May-2016 | Tom Griffiths | Bedford Blues | England | 17-Nov-1995 | 20(166) |
| 01-May-2016 |  Callum Sheedy | Bristol Bears | Wales | 28-Oct-1995 | 20(186) |
| 08-May-2016 | Sebastian Nagle-Taylor | Bedford Blues | England | 16-Jun-1994 | 21(327) |
| 01-May-2016 | Henry Taylor | Bedford Blues | England | 11-Apr-1994 | 22(21) |
| 01-May-2016 | Josh Buggea | Bedford Blues | England | 14-Jan-1994 | 22(108) |
| 01-May-2016 | Harry Wells | Bedford Blues | England | 29-Sep-1993 | 22(215) |
| 01-May-2016 | Peter Lucock | Leeds Tykes | England | 27-Nov-1992 | 23(155) |
| 01-May-2016 |  Andrew Bulumakau | Doncaster Knights | Fiji | 28-Jul-1992 | 23(277) |
| 08-May-2016 | Joel Hodgson | Leeds Tykes | England | 01-Aug-1992 | 23(280) |
| 01-May-2016 | Jonah Holmes | Leeds Tykes | Wales | 24-Jul-1992 | 23(281) |
| 08-May-2016 | Chris Walker | Leeds Tykes | England | 21-Feb-1992 | 24(77) |
| 01-May-2016 | Charles Clare | Bedford Blues | England | 16-Dec-1991 | 24(137) |
| 01-May-2016 | Ben Mosses | Bristol Bears | England | 28-Oct-1991 | 24(186) |
| 01-May-2016 | Marko Mama | Bristol Bears | England | 23-Jul-1991 | 24(283) |
| 01-May-2016 | Oliver Robinson | Bristol Bears | England | 21-Jul-1991 | 24(285) |
| 01-May-2016 | Jake Sharp | Bedford Blues | England | 07-May-1991 | 24(360) |
| 25-May-2016 | Oliver Stedman | Doncaster Knights | England | 20-May-1991 | 25(6) |
| 01-May-2016 | Ben Glynn | Bristol Bears | England | 27-Mar-1991 | 25(36) |
| 01-May-2016 | Jack Wallace | Bristol Bears | England | 22-Jan-1991 | 25(100) |
| 01-May-2016 | Jack Tovey | Bristol Bears | England | 16-Dec-1990 | 25(137) |
| 01-May-2016 | Steffan Jones | Bedford Blues | Wales | 22-Nov-1990 | 25(161) |
| 01-May-2016 | Michael Heaney | Doncaster Knights | Ireland | 05-Nov-1990 | 25(178) |
| 01-May-2016 | Andrew Forsyth | Leeds Tykes | England | 09-Sep-1990 | 25(235) |
| 01-May-2016 | Michael Le Bourgeois | Bedford Blues | Jersey | 29-Jul-1990 | 25(277) |
| 01-May-2016 | Jack O'Connell | Bristol Bears | Ireland | 21-Apr-1990 | 26(11) |
| 01-May-2016 | Will Hurrell | Doncaster Knights | England | 15-Jan-1990 | 26(107) |
| 01-May-2016 | Camilo Parilli | Bedford Blues | England | 20-Dec-1989 | 26(133) |
| 01-May-2016 | Matthew Clark | Doncaster Knights | England | 18-Nov-1989 | 26(165) |
| 01-May-2016 | Matthew Smith | Leeds Tykes | England | 26-Oct-1989 | 26(188) |
| 01-May-2016 | Mark Flanagan | Bedford Blues | Ireland | 02-Aug-1989 | 26(273) |
| 01-May-2016 | Richard Beck | Leeds Tykes | England | 13-Jun-1989 | 26(323) |
| 08-May-2016 | Ralph Nicholas Koster | Bristol Bears | South Africa | 22-Feb-1989 | 27(75) |
| 01-May-2016 | Paul Jarvis | Doncaster Knights | England | 01-Feb-1989 | 27(89) |
| 01-May-2016 | William Cliff | Bristol Bears | England | 17-Oct-1988 | 27(196) |
| 01-May-2016 | Andy Saull | Leeds Tykes | England | 27-Aug-1988 | 27(247) |
| 01-May-2016 | Benjamin Hunter | Doncaster Knights | England | 19-May-1988 | 27(347) |
| 01-May-2016 | Declan Cusack | Doncaster Knights | Ireland | 28-Apr-1988 | 28(3) |
| 01-May-2016 | Ryan Burrows | Leeds Tykes | England | 25-Apr-1988 | 28(6) |
| 01-May-2016 | Ollie Goss | Leeds Tykes | England | 21-Dec-1987 | 28(132) |
| 08-May-2016 | Gareth Maule | Bristol Bears | Wales | 04-Dec-1987 | 28(156) |
| 08-May-2016 | Jack Lam | Bristol Bears | Samoa | 18-Nov-1987 | 28(172) |
| 01-May-2016 | James Phillips | Bristol Bears | England | 27-Sep-1987 | 28(217) |
| 08-May-2016 | Ollie Dodge | Bedford Blues | England | 27-Aug-1987 | 28(255) |
| 01-May-2016 | Charlie Beech | Leeds Tykes | England | 21-Jul-1987 | 28(285) |
| 01-May-2016 | Ross McMillan | Bristol Bears | England | 02-Jun-1987 | 28(334) |
| 01-May-2016 | Alex Shaw | Doncaster Knights | England | 31-Mar-1987 | 29(32) |
| 01-May-2016 | Philip Boulton | Bedford Blues | England | 14-Dec-1986 | 29(139) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).