| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 02-Sep-2000 |  David Waite | Walsall | England | 17-Jul-2000 | 0(47) |
| 02-Sep-2000 | Nick Aldersey | Whitchurch | England | 30-Jul-1981 | 19(34) |
| 30-Sep-2000 |  Gareth Roberts | Sedgley Park | England | 30-Apr-1981 | 19(153) |
| 02-Sep-2000 |  Jody Peacock | Nuneaton | England | 22-Mar-1981 | 19(164) |
| 23-Sep-2000 | Richard Allan | Walsall | England | 24-Mar-1981 | 19(183) |
| 23-Sep-2000 | John Carter | Nuneaton | England | 16-Feb-1981 | 19(219) |
| 30-Sep-2000 | Elijah Sobanjo | Sedgley Park | England | 18-Sep-1980 | 20(12) |
| 14-Oct-2000 | Ryan Banks | Nuneaton | England | 22-Sep-1980 | 20(22) |
| 02-Sep-2000 | Paul Arnold | Sedgley Park | England | 30-Jul-1980 | 20(34) |
| 02-Sep-2000 | Rob Brookshaw | Whitchurch | England | 17-Jun-1980 | 20(77) |
| 28-Oct-2000 |  Andrew Murray | Tynedale | England | 29-Jun-1980 | 20(121) |
| 02-Sep-2000 |  James Ball | Morley | England | 17-Apr-1980 | 20(138) |
| 02-Sep-2000 | Andrew Roberts | Tynedale | England | 08-Mar-1980 | 20(178) |
| 09-Sep-2000 |  Alan Marsh | Liverpool St Helens | England | 05-Mar-1980 | 20(188) |
| 23-Sep-2000 | Andy Losty | Sedgley Park | England | 14-Mar-1980 | 20(193) |
| 09-Sep-2000 | Chris Hares | Whitchurch | England | 31-Jan-1980 | 20(222) |
| 02-Sep-2000 | Michael Jones | Liverpool St Helens | England | 10-Nov-1979 | 20(297) |
| 02-Sep-2000 | Tom Beechy | Walsall | England | 14-Oct-1979 | 20(324) |
| 02-Sep-2000 | Mark Gilbert | Walsall | England | 24-Sep-1979 | 20(344) |
| 02-Sep-2000 | Simon Worsley | Liverpool St Helens | England | 16-Sep-1979 | 20(352) |
| 09-Sep-2000 | Graham Andrews | Aspatria | England | 16-Sep-1979 | 20(359) |
| 14-Oct-2000 | Daniel Clarke | Tynedale | England | 24-Sep-1979 | 21(21) |
| 14-Oct-2000 | Craig Atkinson | Aspatria | England | 21-Sep-1979 | 21(24) |
| 30-Sep-2000 | Tom Ridgway | Walsall | England | 30-Aug-1979 | 21(32) |
| 16-Sep-2000 | Kristofer Bassett | Walsall | England | 08-Aug-1979 | 21(40) |
| 24-Mar-2001 | Steve O'Connor | New Brighton | England | 22-Dec-1979 | 21(93) |
| 02-Sep-2000 | Paul West | Aspatria | England | 21-May-1979 | 21(105) |
| 02-Sep-2000 | Dave McCormack | Sedgley Park | England | 20-May-1979 | 21(106) |
| 24-Mar-2001 | Jay Stirrett | New Brighton | Other | 07-Dec-1979 | 21(108) |
| 14-Oct-2000 | Steve Johnson | New Brighton | England | 27-Mar-1979 | 21(202) |
| 02-Sep-2000 | Adam Butt | New Brighton | England | 12-Feb-1979 | 21(203) |
| 02-Sep-2000 | Stuart Homer | Dudley Kingwinsford | England | 15-Dec-1978 | 21(262) |
| 02-Sep-2000 | Paul Edwards | Whitchurch | England | 09-Dec-1978 | 21(268) |
| 30-Sep-2000 | Chris Stabler | Liverpool St Helens | England | 25-Dec-1978 | 21(280) |
| 16-Sep-2000 | Fraser Harris | Liverpool St Helens | England | 07-Nov-1978 | 21(314) |
| 14-Oct-2000 | Stuart Pearman | Nuneaton | England | 02-Dec-1978 | 21(317) |
| 02-Sep-2000 | Leon Treco | Sedgley Park | England | 27-Sep-1978 | 21(341) |
| 02-Sep-2000 | Simon Lincoln | Bedford Athletic | England | 20-Jul-1978 | 22(45) |
| 02-Sep-2000 | Edward Holmes | Tynedale | England | 27-May-1978 | 22(99) |
| 02-Sep-2000 | Soakai Otuvaka | Tynedale | England | 10-May-1978 | 22(116) |
| 02-Sep-2000 | Shaun Perry | Dudley Kingwinsford | England | 04-May-1978 | 22(122) |
| 23-Sep-2000 | Martin Jackson | Bedford Athletic | England | 18-Feb-1978 | 22(218) |
| 02-Sep-2000 | Matthew Wood | Walsall | England | 28-Jan-1978 | 22(218) |
| 02-Sep-2000 | Sion O'Hare | Sedgley Park | England | 24-Jan-1978 | 22(222) |
| 04-Nov-2000 | Dom Carter | Liverpool St Helens | England | 09-Feb-1978 | 22(269) |
| 02-Sep-2000 | Geoff Mason | Tynedale | England | 11-Nov-1977 | 22(296) |
| 02-Sep-2000 | Ben Shepherd | Walsall | England | 12-Oct-1977 | 22(326) |
| 16-Sep-2000 | Hayden Smith | Whitchurch | England | 21-Oct-1977 | 22(331) |
| 02-Sep-2000 | Andrew Robson | Tynedale | England | 17-Aug-1977 | 23(16) |
| 02-Sep-2000 | Andy Short | Whitchurch | England | 30-Jul-1977 | 23(34) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).