| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 26-Mar-2010 |  Michael Hooper | Brumbies | Australia | 29-Oct-1991 | 18(149) |
| 01-May-2010 | Colby Faingaa | Brumbies | Australia | 31-Mar-1991 | 19(31) |
| 13-Feb-2010 | Wandile Mjekevu | Lions | South Africa | 07-Jan-1991 | 19(37) |
| 15-May-2010 | Jacob Johannes Taute | Lions | South Africa | 21-Mar-1991 | 19(55) |
| 20-Mar-2010 | Patrick Lambie | Sharks | South Africa | 17-Oct-1990 | 19(154) |
| 19-Feb-2010 | Jake Schatz | Queensland Reds | Australia | 25-Jul-1990 | 19(209) |
| 12-Feb-2010 | James O'Connor | Western Force | Australia | 05-Jul-1990 | 19(222) |
| 05-Mar-2010 | Luke Morahan | Queensland Reds | Australia | 13-Apr-1990 | 19(326) |
| 12-Feb-2010 | Matt Toomua | Brumbies | Australia | 02-Jan-1990 | 20(41) |
| 08-May-2010 | Justin Turner | Western Force | Australia | 12-Mar-1990 | 20(57) |
| 13-Feb-2010 | Scott Higginbotham | Queensland Reds | Australia | 05-Sep-1989 | 20(161) |
| 27-Mar-2010 | Ben Whittaker | Western Force | Australia | 10-Oct-1989 | 20(168) |
| 20-Mar-2010 | Robbie Robinson | Highlanders | New Zealand | 22-Aug-1989 | 20(210) |
| 27-Mar-2010 | Rob Horne | New South Wales | Australia | 15-Aug-1989 | 20(224) |
| 15-May-2010 | Deon Helberg | Bulls | South Africa | 27-Sep-1989 | 20(230) |
| 12-Feb-2010 | Dane Haylett Petty | Western Force | Australia | 18-Jun-1989 | 20(239) |
| 13-Feb-2010 | Tim Nanai Williams | Chiefs | Samoa | 12-Jun-1989 | 20(246) |
| 20-Feb-2010 | Kane Douglas | New South Wales | Australia | 01-Jun-1989 | 20(264) |
| 13-Feb-2010 | Rod Davies | Queensland Reds | Australia | 18-May-1989 | 20(271) |
| 08-May-2010 | Phillip van der Walt | Cheetahs | South Africa | 14-Jul-1989 | 20(298) |
| 12-Feb-2010 | Gerhard Jacobus van den Heever | Bulls | Japan | 13-Apr-1989 | 20(305) |
| 14-Mar-2010 | Rob Simmons | Queensland Reds | Australia | 19-Apr-1989 | 20(329) |
| 15-May-2010 | James Slipper | Queensland Reds | Australia | 06-Jun-1989 | 20(343) |
| 13-Feb-2010 | Zac Guildford | Crusaders | New Zealand | 08-Feb-1989 | 21(5) |
| 06-Mar-2010 | Robert Thompson Ebersohn | Cheetahs | South Africa | 23-Feb-1989 | 21(11) |
| 13-Feb-2010 | Kurtley Beale | New South Wales | Australia | 06-Jan-1989 | 21(38) |
| 06-Mar-2010 | Aaron Cruden | Hurricanes | New Zealand | 08-Jan-1989 | 21(57) |
| 07-May-2010 | Andrew Shaw | Queensland Reds | Australia | 02-Feb-1989 | 21(94) |
| 17-Apr-2010 | Nathan Charles | Western Force | Australia | 09-Jan-1989 | 21(98) |
| 06-Mar-2010 | Waltie Vermeulan | Cheetahs | South Africa | 11-Nov-1988 | 21(115) |
| 15-May-2010 | Ben Tapuai | Queensland Reds | Australia | 19-Jan-1989 | 21(116) |
| 20-Feb-2010 | Mitch Inman | Western Force | Australia | 24-Oct-1988 | 21(119) |
| 13-Feb-2010 | Sam Whitelock | Crusaders | New Zealand | 12-Oct-1988 | 21(124) |
| 19-Feb-2010 | Luke Braid | Chiefs | New Zealand | 05-Oct-1988 | 21(137) |
| 26-Feb-2010 | Ryan Crotty | Crusaders | New Zealand | 23-Sep-1988 | 21(156) |
| 13-Feb-2010 | Jackson Willison | Chiefs | New Zealand | 05-Sep-1988 | 21(161) |
| 26-Feb-2010 | Sean Maitland | Crusaders | Scotland | 14-Sep-1988 | 21(165) |
| 06-Mar-2010 | Alapati Leiua | Hurricanes | Samoa | 21-Sep-1988 | 21(166) |
| 13-Feb-2010 | Nasi Manu | Highlanders | Tonga | 15-Aug-1988 | 21(182) |
| 23-Apr-2010 | Toby Smith | Chiefs | New Zealand | 10-Oct-1988 | 21(195) |
| 12-Feb-2010 | Lionel Mapoe | Cheetahs | South Africa | 13-Jul-1988 | 21(214) |
| 27-Feb-2010 | John Hardie | Highlanders | Scotland | 27-Jul-1988 | 21(215) |
| 13-Feb-2010 | Robbie Fruean | Crusaders | New Zealand | 13-Jul-1988 | 21(215) |
| 13-Feb-2010 | Ben Daley | Queensland Reds | Australia | 27-Jun-1988 | 21(231) |
| 13-Feb-2010 | Israel Dagg | Highlanders | New Zealand | 06-Jun-1988 | 21(252) |
| 05-Mar-2010 | Charles Emslie | Lions | South Africa | 14-Jun-1988 | 21(264) |
| 13-Feb-2010 | Dewaldt Otto Duvenage | Western Stormers | South Africa | 22-May-1988 | 21(267) |
| 01-May-2010 | Brackin Karauria-Henry | Brumbies | Australia | 31-Jul-1988 | 21(274) |
| 13-Feb-2010 | Juan De Jongh | Western Stormers | South Africa | 15-May-1988 | 21(274) |
| 05-Mar-2010 | Will Chambers | Queensland Reds | Australia | 26-May-1988 | 21(283) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).