| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 04-Oct-2008 | Rob Milligan | England | 17-Apr-1990 | 18(171) |
| 04-Oct-2008 | Courtney Lawes | England | 23-Feb-1989 | 19(223) |
| 04-Oct-2008 | Chris Ashton | England | 29-Mar-1987 | 21(190) |
| 04-Oct-2008 | Alex Rae | England | 02-Jun-1986 | 22(125) |
| 28-Mar-2009 | Dylan Hartley | England | 24-Mar-1986 | 23(4) |
| 26-Oct-2008 | Ben Foden | England | 22-Jul-1985 | 23(96) |
| 28-Mar-2009 | Joe Ansbro | Scotland | 29-Oct-1985 | 23(150) |
| 04-Oct-2008 | Lee Dickson | England | 29-Mar-1985 | 23(189) |
| 28-Mar-2009 | Petrus Johannes Juandre Kruger | South Africa | 06-Sep-1985 | 23(203) |
| 04-Oct-2008 | Paul Diggin | England | 23-Jan-1985 | 23(254) |
| 26-Oct-2008 | Stephen Myler | England | 21-Jul-1984 | 24(97) |
| 01-Nov-2008 | Mark Hopley | England | 01-May-1984 | 24(184) |
| 04-Oct-2008 | Nils Mordt | England | 05-Dec-1983 | 24(304) |
| 26-Oct-2008 | Christian Day | England | 24-Jun-1983 | 25(125) |
| 04-Oct-2008 | Chris Mayor | England | 19-May-1982 | 26(139) |
| 04-Oct-2008 | Soane Tonga'uiha | Tonga | 21-Jan-1982 | 26(257) |
| 26-Oct-2008 | Roger Wilson | Ireland | 21-Sep-1981 | 27(35) |
| 01-Nov-2008 | James Downey | Ireland | 23-Mar-1981 | 27(223) |
| 04-Oct-2008 | Sean Lamont | Scotland | 15-Jan-1981 | 27(262) |
| 01-Nov-2008 | Euan Murray | Scotland | 07-Aug-1980 | 28(86) |
| 04-Oct-2008 | Ben Lewitt | England | 23-Oct-1979 | 28(347) |
| 28-Mar-2009 | Neil Best | Ireland | 03-Apr-1979 | 29(360) |
| 04-Oct-2008 | Paul Shields | Ireland | 25-Sep-1978 | 30(10) |
| 01-Nov-2008 | Scott Gray | Scotland | 25-Feb-1978 | 30(250) |
| 04-Oct-2008 | Matt Lord | New Zealand | 07-Jan-1978 | 30(271) |
| 01-Nov-2008 | Bruce Reihana | New Zealand | 06-Apr-1976 | 32(209) |
| 04-Oct-2008 | Barry Everitt | Ireland | 09-Mar-1976 | 32(209) |
| 04-Oct-2008 | Barry Stewart | Scotland | 03-Jun-1975 | 33(124) |
| 01-Nov-2008 | Ignacio Fernandez Lobbe | Argentina | 20-Nov-1974 | 33(347) |
| 01-Nov-2008 | Tom Smith | Scotland | 31-Oct-1971 | 37(2) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).