| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 19-Aug-2000 | Matthew Leek | England | 25-Oct-1980 | 19(298) |
| 27-Aug-2000 | Richard Birkett | England | 01-Oct-1979 | 20(331) |
| 06-Sep-2000 | Paul Sampson | England | 12-Jul-1977 | 23(56) |
| 19-Aug-2000 | Joe Worsley | England | 14-Jun-1977 | 23(66) |
| 19-Aug-2000 | Martyn Wood | England | 25-Apr-1977 | 23(116) |
| 19-Aug-2000 | Josh Lewsey | England | 30-Nov-1976 | 23(262) |
| 19-Aug-2000 | Joe Beardshaw | England | 30-Oct-1976 | 23(293) |
| 19-Aug-2000 | Fraser Waters | England | 31-Mar-1976 | 24(141) |
| 19-Aug-2000 | Phil Greening | England | 03-Oct-1975 | 24(321) |
| 18-Nov-2000 | Andy Le Chevalier | England | 05-Nov-1975 | 25(14) |
| 27-Aug-2000 | Mark Denney | England | 25-Jan-1975 | 25(215) |
| 01-Oct-2000 | Trevor Leota | Samoa | 08-Feb-1975 | 25(236) |
| 01-Oct-2000 | Alex King | England | 17-Jan-1975 | 25(258) |
| 19-Aug-2000 | John Ufton | England | 31-Jan-1974 | 26(201) |
| 19-Aug-2000 | Will Green | England | 25-Oct-1973 | 26(299) |
| 19-Aug-2000 | Simon Shaw | England | 01-Sep-1973 | 26(353) |
| 19-Aug-2000 | Andy Reed | Scotland | 07-Jun-1973 | 27(73) |
| 27-Aug-2000 | Shane Roiser | England | 07-Jun-1973 | 27(81) |
| 19-Aug-2000 | Rob Henderson | Ireland | 27-Oct-1972 | 27(296) |
| 19-Aug-2000 | Darren Molloy | England | 31-Aug-1972 | 27(353) |
| 09-Sep-2000 | Laurence Scrase | England | 10-Sep-1972 | 27(364) |
| 06-Sep-2000 | Lawrence Dallaglio | England | 10-Aug-1972 | 28(27) |
| 26-Nov-2000 | Mark Lock | England | 22-Sep-1972 | 28(65) |
| 19-Aug-2000 | Kenny Logan | Scotland | 03-Apr-1972 | 28(138) |
| 10-Feb-2001 | Dugald Macer | * | 03-Jun-1972 | 28(252) |
| 19-Aug-2000 | Paul Volley | England | 02-Nov-1971 | 28(291) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).