| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 03-Jan-2021 | George Martin | England | 18-Jun-2001 | 19(199) |
| 27-Feb-2021 | Daniel Kelly | England | 16-Jun-2001 | 19(256) |
| 13-Feb-2021 | Jack van Poortvliet | England | 15-May-2001 | 19(274) |
| 21-Nov-2020 | Freddie Steward | England | 05-Dec-2000 | 19(351) |
| 27-Feb-2021 | Oliver Chessum | England | 06-Sep-2000 | 20(174) |
| 27-Feb-2021 | Cameron Henderson | Scotland | 13-Jan-2000 | 21(46) |
| 21-Nov-2020 | Tommy Reffell | Wales | 27-Apr-1999 | 21(209) |
| 13-Feb-2021 | Joseph Heyes | England | 13-Apr-1999 | 21(307) |
| 21-Nov-2020 | Harry Potter | England | 15-Dec-1997 | 22(342) |
| 05-Dec-2020 | Harry Simmons | England | 25-Nov-1997 | 23(10) |
| 21-Nov-2020 | Guy Porter | England | 23-Jan-1997 | 23(302) |
| 07-May-2021 | Jonny McPhillips | Ireland | 13-Mar-1997 | 24(55) |
| 21-Nov-2020 | Jasper Wiese | South Africa | 21-Oct-1995 | 25(32) |
| 21-Nov-2020 | Hanro Liebenberg | South Africa | 10-Oct-1995 | 25(43) |
| 03-Jan-2021 | Ellis Genge | England | 16-Feb-1995 | 25(322) |
| 21-Nov-2020 | Zack Henry | England | 01-Oct-1994 | 26(52) |
| 21-Nov-2020 | Facundo Gigena | Argentina | 15-Sep-1994 | 26(68) |
| 29-Nov-2020 | Cyle Brink | South Africa | 16-Jan-1994 | 26(318) |
| 05-Dec-2020 | Nephi Leatigaga | Samoa | 05-Dec-1993 | 27(0) |
| 21-Nov-2020 | Harry Wells | England | 29-Sep-1993 | 27(53) |
| 06-Feb-2021 | Julian Montoya | Argentina | 29-Oct-1993 | 27(100) |
| 03-Jan-2021 | George Ford | England | 16-Mar-1993 | 27(293) |
| 29-Jan-2021 | Luan de Bruin | South Africa | 13-Feb-1993 | 27(350) |
| 06-Feb-2021 | Tomas Lavanini | Argentina | 22-Jan-1993 | 28(15) |
| 21-Nov-2020 | Kobus van Wyk | South Africa | 22-Jan-1992 | 28(304) |
| 20-Mar-2021 | Charles Clare | England | 16-Dec-1991 | 29(95) |
| 21-Nov-2020 | Jacob Johannes Taute | South Africa | 21-Mar-1991 | 29(246) |
| 29-Nov-2020 | Ryan Bower | England | 25-Feb-1991 | 29(278) |
| 29-Jan-2021 | Matias Moroni | Argentina | 29-Mar-1991 | 29(307) |
| 29-Nov-2020 | Matt Scott | Scotland | 30-Sep-1990 | 30(61) |
| 21-Nov-2020 | Callum Green | England | 15-Jul-1990 | 30(130) |
| 24-Apr-2021 | Luke Wallace | England | 02-Oct-1990 | 30(205) |
| 03-Jan-2021 | Ben Youngs | England | 05-Sep-1989 | 31(120) |
| 13-Feb-2021 | Kini Murimurivalu | Fiji | 15-May-1989 | 31(275) |
| 27-Feb-2021 | Joaquin Diaz Bonilla | Argentina | 12-Apr-1989 | 31(321) |
| 03-Jan-2021 | Nemani Nadolo | Fiji | 31-Jan-1988 | 32(338) |
| 21-Nov-2020 | Dan Cole | England | 09-May-1987 | 33(197) |
| 21-Nov-2020 | Tom Youngs | England | 28-Jan-1987 | 33(298) |
| 21-Nov-2020 | Richard Wigglesworth | England | 09-Jun-1983 | 37(166) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).