| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 14-Feb-2010 | Dan Cole | England | 09-May-1987 | 22(282) |
| 20-Mar-2010 | Chris Ashton | England | 29-Mar-1987 | 22(357) |
| 06-Feb-2010 | Danny Care | England | 02-Jan-1987 | 23(35) |
| 06-Feb-2010 | Dylan Hartley | England | 24-Mar-1986 | 23(319) |
| 06-Feb-2010 | Matthew Tait | England | 06-Feb-1986 | 24(0) |
| 06-Feb-2010 | Toby Flood | England | 08-Aug-1985 | 24(182) |
| 20-Mar-2010 | Ben Foden | England | 22-Jul-1985 | 24(241) |
| 06-Feb-2010 | Dave Wilson | England | 09-Apr-1985 | 24(303) |
| 06-Feb-2010 | James Haskell | England | 02-Apr-1985 | 24(310) |
| 06-Feb-2010 | Delon Armitage | England | 15-Dec-1983 | 26(54) |
| 06-Feb-2010 | Ugo Monye | England | 13-Apr-1983 | 26(300) |
| 13-Mar-2010 | Louis Deacon | England | 07-Oct-1980 | 29(157) |
| 14-Feb-2010 | Riki Flutey | England | 10-Feb-1980 | 30(5) |
| 06-Feb-2010 | Mark Cueto | England | 26-Dec-1979 | 30(43) |
| 06-Feb-2010 | Steve Borthwick | England | 12-Oct-1979 | 30(118) |
| 06-Feb-2010 | Jonny Wilkinson | England | 25-May-1979 | 30(258) |
| 06-Feb-2010 | Tim Payne | England | 29-Apr-1979 | 30(284) |
| 20-Mar-2010 | Mike Tindall | England | 18-Oct-1978 | 31(154) |
| 06-Feb-2010 | Nick Easter | England | 15-Aug-1978 | 31(175) |
| 06-Feb-2010 | Lewis Moody | England | 12-Jun-1978 | 31(239) |
| 13-Mar-2010 | Joe Worsley | England | 14-Jun-1977 | 32(272) |
| 06-Feb-2010 | Simon Shaw | England | 01-Sep-1973 | 36(158) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).