| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 28-Oct-1999 |  Frank Bunce | Bristol Bears | New Zealand | 04-Feb-1962 | 37(266) |
| 12-Mar-2000 |  Alan Yates | Newcastle Falcons | England | 05-Jan-1963 | 37(67) |
| 11-Sep-1999 | Rob Andrew | Newcastle Falcons | England | 18-Feb-1963 | 36(205) |
| 21-May-2000 |  Victor Ubogu | Bath | England | 08-Sep-1964 | 35(256) |
| 29-Dec-1999 |  Dave Baldwin | Sale Sharks | England | 03-Sep-1964 | 35(117) |
| 21-May-2000 |  Zinzan Brooke | Harlequins | New Zealand | 14-Feb-1965 | 35(97) |
| 21-May-2000 |  Allan Bateman | Northampton Saints | Wales | 06-Mar-1965 | 35(77) |
| 20-May-2000 |  Kevin Putt | London Irish | Ireland | 28-Jul-1965 | 34(297) |
| 20-May-2000 |  Andrew Deacon | Gloucester | England | 31-Jul-1965 | 34(294) |
| 21-May-2000 | Henry Honiball | Bristol Bears | South Africa | 01-Dec-1965 | 34(172) |
| 14-May-2000 | Steve Diamond | Sale Sharks | England | 03-Feb-1966 | 34(101) |
| 06-May-2000 | Jim Mallinder | Sale Sharks | England | 16-Mar-1966 | 34(52) |
| 20-May-2000 | Steve Bachop | London Irish | Samoa | 02-Apr-1966 | 34(49) |
| 12-Feb-2000 | George Graham | Newcastle Falcons | Scotland | 19-Jan-1966 | 34(24) |
| 29-Dec-1999 | Will Carling | Harlequins | England | 12-Dec-1965 | 34(17) |
| 30-Dec-1999 | Jonathan Callard | Bath | England | 01-Jan-1966 | 33(363) |
| 25-Mar-2000 |  Darren Garforth | Leicester Tigers | England | 09-Apr-1966 | 33(351) |
| 14-May-2000 | Dean Ryan | Bristol Bears | England | 22-Jun-1966 | 33(327) |
| 29-Apr-2000 | Alan Charron | Bristol Bears | Canada | 27-Jul-1966 | 33(277) |
| 21-May-2000 | Garry Pagel | Northampton Saints | South Africa | 17-Sep-1966 | 33(247) |
| 14-May-2000 | Dawie Theron | Sale Sharks | South Africa | 15-Sep-1966 | 33(242) |
| 21-May-2000 | Gary Armstrong | Newcastle Falcons | Scotland | 30-Sep-1966 | 33(234) |
| 06-May-2000 | Chris Sheasby | Harlequins | England | 30-Nov-1966 | 33(158) |
| 21-May-2000 |  Thierry Lacroix | Saracens | France | 02-Mar-1967 | 33(81) |
| 20-May-2000 | Adrian Powles | Gloucester | England | 14-Mar-1967 | 33(68) |
| 20-May-2000 | Ian Jones | Gloucester | New Zealand | 17-Apr-1967 | 33(34) |
| 21-May-2000 | Paul Graham | Harlequins | Wales | 28-Apr-1967 | 33(24) |
| 14-May-2000 | Alastair Murdoch | Bedford Blues | England | 09-May-1967 | 33(6) |
| 30-Dec-1999 | Francois Pienaar | Saracens | South Africa | 02-Jan-1967 | 32(362) |
| 21-May-2000 | Gareth Rees | Harlequins | Canada | 30-Jun-1967 | 32(326) |
| 08-Apr-2000 | John Schuster | Harlequins | Other | 30-Jun-1967 | 32(283) |
| 22-Apr-2000 | Dorian West | Leicester Tigers | England | 05-Oct-1967 | 32(200) |
| 21-May-2000 | Simon Mitchell | Harlequins | England | 23-Nov-1967 | 32(180) |
| 06-May-2000 | Jon Preston | Bath | New Zealand | 15-Nov-1967 | 32(173) |
| 04-Dec-1999 | Serge Simon | Gloucester | France | 03-Jul-1967 | 32(154) |
| 25-Mar-2000 | Kevin Nepia | Harlequins | New Zealand | 27-Dec-1967 | 32(89) |
| 21-May-2000 | Dave Lougheed | Leicester Tigers | Canada | 11-Apr-1968 | 32(40) |
| 21-May-2000 | Ben Clarke | Bath | England | 15-Apr-1968 | 32(36) |
| 08-Apr-2000 | Richard Tombs | Gloucester | Australia | 01-Apr-1968 | 32(7) |
| 25-Jan-2000 | Chris Wright | Harlequins | England | 08-Feb-1968 | 31(352) |
| 17-May-2000 |  Mark Weedon | London Wasps | New Zealand | 13-Jul-1968 | 31(308) |
| 10-Oct-1999 | David Pears | Harlequins | England | 06-Dec-1967 | 31(308) |
| 06-May-2000 | Martin Haag | Bath | England | 28-Jul-1968 | 31(283) |
| 06-May-2000 | Jason Leonard | Harlequins | England | 14-Aug-1968 | 31(265) |
| 20-May-2000 | Jarrod Cunningham | London Irish | * | 07-Sep-1968 | 31(255) |
| 21-May-2000 | Phil De Glanville | Bath | England | 01-Oct-1968 | 31(233) |
| 30-Apr-2000 | Phil Winstanley | Sale Sharks | England | 16-Sep-1968 | 31(226) |
| 22-Apr-2000 | Pat Lam | Northampton Saints | Samoa | 29-Sep-1968 | 31(205) |
| 30-Dec-1999 | Warwick Waugh | Bath | Australia | 17-Jul-1968 | 31(165) |
| 30-Apr-2000 | Junior Paramore | Gloucester | Samoa | 18-Nov-1968 | 31(163) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).