| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 14-Apr-2001 |  Dave Baldwin | Sale Sharks | England | 03-Sep-1964 | 36(223) |
| 31-Mar-2001 |  Allan Bateman | Northampton Saints | Wales | 06-Mar-1965 | 36(25) |
| 16-Apr-2001 |  Andrew Deacon | Gloucester | England | 31-Jul-1965 | 35(260) |
| 10-Apr-2001 |  David Scully | Rotherham Titans | England | 07-Aug-1965 | 35(247) |
| 30-Sep-2000 |  Kevin Ellis | London Irish | Wales | 29-Apr-1965 | 35(155) |
| 26-Nov-2000 |  Jeremy Guscott | Bath | England | 07-Jul-1965 | 35(143) |
| 10-Apr-2001 |  George Graham | Newcastle Falcons | Scotland | 19-Jan-1966 | 35(82) |
| 14-Apr-2001 | Mike Umaga | Rotherham Titans | Samoa | 19-Feb-1966 | 35(55) |
| 14-Apr-2001 |  Darren Garforth | Leicester Tigers | England | 09-Apr-1966 | 35(6) |
| 16-Apr-2001 | Garry Pagel | Northampton Saints | South Africa | 17-Sep-1966 | 34(212) |
| 30-Sep-2000 | Jim Mallinder | Sale Sharks | England | 16-Mar-1966 | 34(199) |
| 15-Apr-2001 | Gary Armstrong | Newcastle Falcons | Scotland | 30-Sep-1966 | 34(198) |
| 10-Apr-2001 | Chris Sheasby | London Irish | England | 30-Nov-1966 | 34(132) |
| 30-Sep-2000 |  Dean Ryan | Bristol Bears | England | 22-Jun-1966 | 34(101) |
| 14-Apr-2001 |  David Wilson | Harlequins | Australia | 04-Jan-1967 | 34(101) |
| 31-Mar-2001 | Ian Jones | Gloucester | New Zealand | 17-Apr-1967 | 33(349) |
| 30-Sep-2000 | Adrian Powles | Gloucester | England | 14-Mar-1967 | 33(201) |
| 26-Nov-2000 | Terry Garnett | Rotherham Titans | England | 10-May-1967 | 33(201) |
| 14-Apr-2001 | Dorian West | Leicester Tigers | England | 05-Oct-1967 | 33(192) |
| 14-Apr-2001 | Jon Preston | Bath | New Zealand | 15-Nov-1967 | 33(151) |
| 17-Sep-2000 | Serge Simon | Gloucester | France | 03-Jul-1967 | 33(77) |
| 14-Apr-2001 | Ben Clarke | Bath | England | 15-Apr-1968 | 32(364) |
| 10-Apr-2001 | Jeff Fahrenson | London Irish | * | 21-Apr-1968 | 32(354) |
| 14-Apr-2001 | Jason Leonard | Harlequins | England | 14-Aug-1968 | 32(243) |
| 17-Mar-2001 | Martin Haag | Bath | England | 28-Jul-1968 | 32(232) |
| 16-Apr-2001 | Jarrod Cunningham | London Irish | * | 07-Sep-1968 | 32(221) |
| 14-Apr-2001 | Phil De Glanville | Bath | England | 01-Oct-1968 | 32(195) |
| 17-Mar-2001 | Pat Lam | Northampton Saints | Samoa | 29-Sep-1968 | 32(169) |
| 09-Sep-2000 | Dave Lougheed | Gloucester | Canada | 11-Apr-1968 | 32(151) |
| 31-Mar-2001 | Junior Paramore | Gloucester | Samoa | 18-Nov-1968 | 32(133) |
| 14-Apr-2001 | Neil Back | Leicester Tigers | England | 16-Jan-1969 | 32(88) |
| 27-Dec-2000 | Craig Chalmers | Harlequins | Scotland | 15-Oct-1968 | 32(73) |
| 15-Apr-2001 |  Bill Davison | Saracens | England | 08-Apr-1969 | 32(7) |
| 06-Sep-2000 | Phil Winstanley | Sale Sharks | England | 16-Sep-1968 | 31(355) |
| 14-Mar-2001 | Rob Hardwick | London Irish | England | 29-Mar-1969 | 31(350) |
| 27-Dec-2000 | Eric Peters | Harlequins | Scotland | 28-Jan-1969 | 31(334) |
| 10-Apr-2001 | Adrian Olver | Harlequins | England | 02-Jun-1969 | 31(313) |
| 16-Dec-2000 | Harvey Thorneycroft | Northampton Saints | Other | 22-Feb-1969 | 31(297) |
| 10-Apr-2001 | Simon Halford | London Irish | * | 26-Jun-1969 | 31(289) |
| 18-Mar-2001 | Leighton Gerrard | Bristol Bears | * | 10-Jun-1969 | 31(281) |
| 16-Apr-2001 | Tim Rodber | Northampton Saints | England | 12-Jul-1969 | 31(278) |
| 10-Apr-2001 | Jason Wright | London Irish | New Zealand | 08-Jul-1969 | 31(276) |
| 14-Apr-2001 | Apollo Perelini | Sale Sharks | Samoa | 16-Jul-1969 | 31(273) |
| 15-Apr-2001 | Ross Nesdale | Newcastle Falcons | Ireland | 30-Jul-1969 | 31(260) |
| 15-Apr-2001 | Richard Arnold | Newcastle Falcons | * | 16-Aug-1969 | 31(242) |
| 24-Mar-2001 | Rhodri Latham | Rotherham Titans | * | 01-Aug-1969 | 31(236) |
| 14-Apr-2001 | Michael Schmid | Rotherham Titans | Canada | 28-Nov-1969 | 31(138) |
| 16-Apr-2001 | Neal Hatley | London Irish | England | 23-Dec-1969 | 31(115) |
| 31-Mar-2001 | Glenn Gelderbloom | Leicester Tigers | South Africa | 11-Dec-1969 | 31(110) |
| 14-Apr-2001 | Garrick Morgan | Harlequins | Australia | 25-Jan-1970 | 31(80) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).