| MD | Players | Nationality | DOB | A(D) |
|---|---|---|---|---|
| 07-May-2016 | Neil de Koch | South Africa | 20-Nov-1978 | 37(169) |
| 07-May-2016 | Charlie Hodgson | England | 12-Nov-1980 | 35(177) |
| 28-Nov-2015 | Ernst Joubert | South Africa | 05-Aug-1980 | 35(115) |
| 07-May-2016 | Petrus Du Plessis | England | 31-May-1981 | 34(342) |
| 16-Apr-2016 | Schalk Brits | South Africa | 16-May-1981 | 34(336) |
| 07-May-2016 | Kelly Brown | Scotland | 08-Jun-1982 | 33(334) |
| 07-May-2016 | James Hamilton | Scotland | 17-Nov-1982 | 33(172) |
| 01-May-2016 | Richard Wigglesworth | England | 09-Jun-1983 | 32(327) |
| 01-May-2016 | Jacques Burger | Namibia | 29-Jul-1983 | 32(277) |
| 07-May-2016 | Chris Wyles | United States | 13-Sep-1983 | 32(237) |
| 07-May-2016 | Marcelo Bosch | Argentina | 07-Jan-1984 | 32(121) |
| 20-Mar-2016 | Nils Mordt | England | 05-Dec-1983 | 32(106) |
| 12-Mar-2016 | Hayden Smith | United States | 10-Apr-1985 | 30(337) |
| 20-Mar-2016 | Alistair John Hargreaves | South Africa | 29-Apr-1986 | 29(326) |
| 01-May-2016 | Bradley Barritt | England | 07-Aug-1986 | 29(268) |
| 20-Feb-2016 | Rhys Gill | Wales | 30-Oct-1986 | 29(113) |
| 07-May-2016 | Chris Ashton | England | 29-Mar-1987 | 29(40) |
| 01-May-2016 | Michael Rhodes | South Africa | 19-Dec-1987 | 28(134) |
| 07-May-2016 | Alex Goode | England | 07-May-1988 | 28(0) |
| 20-Mar-2016 | Juan Figallo | Argentina | 25-Mar-1988 | 27(360) |
| 07-May-2016 | Samu Vunisa | Italy | 19-Aug-1988 | 27(261) |
| 17-Oct-2015 | Tim Streather | England | 14-Jun-1988 | 27(124) |
| 07-May-2016 | Richard Barrington | England | 24-Sep-1989 | 26(226) |
| 16-Apr-2016 | Duncan Taylor | Scotland | 05-Sep-1989 | 26(224) |
| 20-Mar-2016 | Michael Ellery | England | 08-Sep-1989 | 26(194) |
| 01-Apr-2016 | Will Fraser | England | 29-Oct-1989 | 26(155) |
| 07-May-2016 | George Kruis | England | 22-Feb-1990 | 26(75) |
| 01-May-2016 | Jared Saunders | England | 18-Sep-1990 | 25(226) |
| 07-May-2016 | Jamie George | England | 20-Oct-1990 | 25(200) |
| 07-May-2016 | Jackson Wray | England | 10-Nov-1990 | 25(179) |
| 12-Mar-2016 | Jared Saunders | England | 18-Sep-1990 | 25(176) |
| 16-Apr-2016 | Makovina Vunipola | England | 13-Jan-1991 | 25(94) |
| 01-Apr-2016 | Owen Farrell | England | 21-Sep-1991 | 24(193) |
| 01-May-2016 | Ben Ransom | England | 16-Feb-1992 | 24(75) |
| 12-Mar-2016 | Ben Spencer | England | 13-Jul-1992 | 23(242) |
| 16-Apr-2016 | Billy Vunipola | England | 03-Nov-1992 | 23(164) |
| 01-May-2016 | Maro Itoje | England | 28-Oct-1994 | 21(186) |
| 07-May-2016 | Nick Tompkins | Wales | 16-Feb-1995 | 21(81) |
| 01-May-2016 | Titi Lamositele | United States | 11-Feb-1995 | 21(80) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).