| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 06-Jan-2001 |  Tom Youngs | North Walsham | England | 28-Jan-1987 | 13(344) |
| 21-Apr-2001 |  Luke Greatrix | Launceston | England | 01-Oct-1986 | 14(203) |
| 27-Jan-2001 |  Chris Bell | Plymouth Albion | England | 07-Jul-1983 | 17(205) |
| 25-Nov-2000 |  Luke Collins | Redruth | England | 30-Jun-1982 | 18(149) |
| 09-Sep-2000 |  Pat Sykes | Westcombe Park | England | 12-Feb-1982 | 18(210) |
| 09-Sep-2000 | Tim Mathias | Weston super Mare | England | 12-Jun-1981 | 19(89) |
| 13-Jan-2001 | Anthony Birley | Launceston | Other | 04-Oct-1981 | 19(101) |
| 30-Sep-2000 |  James Hawken | Cornish Pirates | * | 18-Apr-1981 | 19(165) |
| 02-Sep-2000 | Paul Thirlby | Redruth | England | 30-Jan-1981 | 19(215) |
| 30-Sep-2000 |  Joe Bonner | Blackheath | England | 03-Jan-1981 | 19(270) |
| 04-Nov-2000 | Paul Belcher | Westcombe Park | * | 30-Sep-1980 | 20(35) |
| 30-Sep-2000 | Matt Vick | Westcombe Park | England | 15-Jul-1980 | 20(77) |
| 23-Sep-2000 |  Adam Holloway | Reading | England | 05-Jun-1980 | 20(110) |
| 09-Sep-2000 | Stefan Bober | Westcombe Park | * | 11-May-1980 | 20(121) |
| 23-Sep-2000 | Oliver Pritchard | Clifton | England | 20-Apr-1980 | 20(156) |
| 02-Sep-2000 | Andrew Grierson | Reading | * | 23-Nov-1979 | 20(284) |
| 02-Sep-2000 | Matt Senior | Reading | * | 25-Oct-1979 | 20(313) |
| 16-Dec-2000 | Sid Claffey | Basingstoke | Other | 15-Jan-1980 | 20(336) |
| 02-Sep-2000 | Simon Dyson | Reading | * | 24-Sep-1979 | 20(344) |
| 21-Apr-2001 | Sean Lynch | Reading | Other | 16-Mar-1980 | 21(36) |
| 28-Apr-2001 | Jason Bolt | Launceston | Other | 13-Mar-1980 | 21(46) |
| 17-Feb-2001 | Dan Clackworthy | Cornish Pirates | England | 18-Dec-1979 | 21(62) |
| 02-Sep-2000 | Huw Conquer | Reading | * | 21-May-1979 | 21(105) |
| 02-Dec-2000 | David Pascoe | Cornish Pirates | Other | 12-Aug-1979 | 21(113) |
| 23-Sep-2000 | Matt Johnston | Reading | Scotland | 01-Jun-1979 | 21(115) |
| 02-Sep-2000 | Peter Redgrave | Cornish Pirates | England | 25-Mar-1979 | 21(162) |
| 23-Dec-2000 | Robin Campbell | Westcombe Park | Other | 03-Jul-1979 | 21(174) |
| 16-Sep-2000 | Joe Bearman | Cornish Pirates | England | 28-Feb-1979 | 21(201) |
| 02-Sep-2000 | Chris Holder | Cheltenham | England | 08-Dec-1978 | 21(269) |
| 09-Sep-2000 | Matt Jarvis | Cornish Pirates | England | 18-Oct-1978 | 21(327) |
| 28-Oct-2000 | Simon Wightwick | Reading | * | 15-Sep-1978 | 22(44) |
| 16-Sep-2000 | Justin Smith | Blackheath | * | 18-Jul-1978 | 22(61) |
| 28-Oct-2000 | Jon Salisbury | Reading | * | 24-Aug-1978 | 22(66) |
| 02-Sep-2000 | Simon Elkinson | Blackheath | England | 20-May-1978 | 22(106) |
| 02-Sep-2000 | Neil Bayliss | Cheltenham | * | 28-Mar-1978 | 22(159) |
| 02-Sep-2000 | Steve Evans | Cornish Pirates | England | 25-Mar-1978 | 22(162) |
| 02-Sep-2000 | Matt Collie | Tabard | England | 09-Jan-1978 | 22(237) |
| 02-Sep-2000 | Dan Ward-Smith | Plymouth Albion | England | 02-Jan-1978 | 22(244) |
| 23-Sep-2000 | Ross Stewart | Reading | * | 21-Jan-1978 | 22(246) |
| 02-Sep-2000 | David Glendinning | Blackheath | England | 05-Dec-1977 | 22(272) |
| 02-Sep-2000 | Alan Paver | Plymouth Albion | England | 28-Nov-1977 | 22(279) |
| 16-Sep-2000 | Rhys Powell | Blackheath | Wales | 05-Dec-1977 | 22(286) |
| 02-Sep-2000 | Dean Sole | Westcombe Park | * | 16-Nov-1977 | 22(291) |
| 02-Sep-2000 | Hugh Colvin | Westcombe Park | * | 01-Oct-1977 | 22(337) |
| 02-Sep-2000 | Tom Barlow | Plymouth Albion | England | 28-Sep-1977 | 22(340) |
| 25-Nov-2000 | Qin Wiseman | Westcombe Park | Other | 26-Oct-1977 | 23(30) |
| 02-Sep-2000 | Nigel Cane | Plymouth Albion | England | 19-Jun-1977 | 23(75) |
| 16-Sep-2000 | Chris Randall | Clifton | * | 14-Jun-1977 | 23(94) |
| 02-Sep-2000 | Alyn Franklin | Clifton | * | 25-Feb-1977 | 23(189) |
| 02-Sep-2000 | Kevin Mullen | Blackheath | * | 19-Feb-1977 | 23(195) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).