| MD | Players | Club | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 05-Mar-2016 |  Victor Matfield | Northampton Saints | South Africa | 11-May-1977 | 38(299) |
| 07-May-2016 |  Peter Stringer | Sale Sharks | Ireland | 13-Dec-1977 | 38(146) |
| 07-May-2016 |  Nick Easter | Harlequins | England | 15-Aug-1978 | 37(266) |
| 07-May-2016 |  Neil de Koch | Saracens | South Africa | 20-Nov-1978 | 37(169) |
| 07-May-2016 |  Donncha O'Callaghan | Worcester Warriors | Ireland | 24-Mar-1979 | 37(45) |
| 02-Jan-2016 |  Seremaia Bai | Leicester Tigers | Fiji | 04-Jan-1979 | 36(363) |
| 04-Mar-2016 |  Andy Goode | Newcastle Falcons | England | 03-Apr-1980 | 35(336) |
| 01-May-2016 |  Carlo Festuccia | London Wasps | Italy | 20-Jun-1980 | 35(316) |
| 01-May-2016 | George Smith | London Wasps | Australia | 17-Jul-1980 | 35(289) |
| 07-May-2016 | Nick Evans | Harlequins | New Zealand | 14-Aug-1980 | 35(267) |
| 07-May-2016 | Charlie Hodgson | Saracens | England | 12-Nov-1980 | 35(177) |
| 17-Apr-2016 |  Tom Court | London Irish | Ireland | 06-Nov-1980 | 35(163) |
| 28-Nov-2015 | Ernst Joubert | Saracens | South Africa | 05-Aug-1980 | 35(115) |
| 07-May-2016 | Eifion Roberts | Sale Sharks | Wales | 13-Feb-1981 | 35(84) |
| 28-Feb-2016 | Jean de Villiers | Leicester Tigers | South Africa | 24-Feb-1981 | 35(4) |
| 28-Feb-2016 | Adam Jones | Harlequins | Wales | 08-Mar-1981 | 34(357) |
| 07-May-2016 | Petrus Du Plessis | Saracens | England | 31-May-1981 | 34(342) |
| 16-Apr-2016 | Schalk Brits | Saracens | South Africa | 16-May-1981 | 34(336) |
| 02-Jan-2016 | Daniel Braid | Sale Sharks | New Zealand | 13-Feb-1981 | 34(323) |
| 06-Feb-2016 | James Downey | London Wasps | Ireland | 23-Mar-1981 | 34(320) |
| 05-Dec-2015 | Matias Aguero | Leicester Tigers | Italy | 13-Feb-1981 | 34(295) |
| 07-Nov-2015 | Alesana Tuilagi | Newcastle Falcons | Samoa | 24-Feb-1981 | 34(256) |
| 07-May-2016 | Phil Dowson | Worcester Warriors | England | 01-Oct-1981 | 34(219) |
| 04-Mar-2016 | Scott Lawson | Newcastle Falcons | Scotland | 28-Sep-1981 | 34(158) |
| 07-May-2016 | Rob Vickers | Newcastle Falcons | England | 02-Dec-1981 | 34(157) |
| 18-Mar-2016 |  Stuart Hooper | Bath | England | 18-Nov-1981 | 34(121) |
| 01-May-2016 | Mouritz Botha | Newcastle Falcons | England | 29-Jan-1982 | 34(93) |
| 07-May-2016 | Nili Latu | Newcastle Falcons | Tonga | 19-Feb-1982 | 34(78) |
| 17-Apr-2016 | Sam Tuitupou | Sale Sharks | New Zealand | 01-Feb-1982 | 34(76) |
| 07-May-2016 | Kelly Brown | Saracens | Scotland | 08-Jun-1982 | 33(334) |
| 07-May-2016 | Mike Delany | Newcastle Falcons | New Zealand | 15-Jun-1982 | 33(327) |
| 03-Apr-2016 | Kahn Fotuali'i | Northampton Saints | Samoa | 22-May-1982 | 33(317) |
| 07-Nov-2015 | Kane Thompson | Newcastle Falcons | Samoa | 09-Jan-1982 | 33(302) |
| 07-May-2016 |  Damian Welch | Exeter Chiefs | Wales | 28-Jul-1982 | 33(284) |
| 05-Mar-2016 | Chris Cusiter | Sale Sharks | Scotland | 13-Jun-1982 | 33(266) |
| 10-Jan-2016 | Ben Jacobs | London Wasps | Australia | 17-May-1982 | 33(238) |
| 19-Mar-2016 | Karl Dickson | Harlequins | England | 02-Aug-1982 | 33(230) |
| 23-Apr-2016 | Mark Easter | Sale Sharks | England | 19-Oct-1982 | 33(187) |
| 07-May-2016 | James Hamilton | Saracens | Scotland | 17-Nov-1982 | 33(172) |
| 01-Jan-2016 | Tom, Johnson | Exeter Chiefs | England | 16-Jul-1982 | 33(169) |
| 07-May-2016 | Lorenzo Cittadini | London Wasps | Italy | 17-Dec-1982 | 33(142) |
| 30-Apr-2016 | Marcos Ayerza | Leicester Tigers | Argentina | 12-Jan-1983 | 33(109) |
| 02-Apr-2016 | Allister Hogg | Newcastle Falcons | Scotland | 20-Jan-1983 | 33(73) |
| 20-Mar-2016 |  Nick Wood | Gloucester | England | 09-Jan-1983 | 33(71) |
| 10-Jan-2016 | Sailosi Tagicakibau | London Wasps | Samoa | 14-Nov-1982 | 33(57) |
| 07-May-2016 | Rob Hawkins | Newcastle Falcons | England | 14-Apr-1983 | 33(24) |
| 07-May-2016 | Thomas Waldron | Exeter Chiefs | England | 28-Apr-1983 | 33(10) |
| 05-Mar-2016 | Tito Tebaldi | Harlequins | Italy | 24-Mar-1983 | 32(347) |
| 07-Nov-2015 | George Skivington | London Irish | England | 03-Dec-1982 | 32(339) |
| 01-May-2016 | Richard Wigglesworth | Saracens | England | 09-Jun-1983 | 32(327) |
MD = Match date,
DOB = Date of birth,
A(D) = Age(days).